Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH
    • Molecular weight
      20 kDa including tags
    • Amino acids
      1 to 158
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab168008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    > 60,000 unit/mg of protein. One unit is defined as the amount of enzyme that will hydrolyze 1 nmole of p-nitrophenyl phosphate per minute at 37°C in MES pH 5.0 using 10 mM of substrate.
  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    >95% by SDS-PAGE .

  • Form
    Liquid
  • Additional notes
    Centrifuge the vial prior to opening. > 60,000 unit/mg of protein. One unit is defined as the amount of enzyme that will hydrolyze 1 nmole of p-nitrophenyl phosphate per minute at 37°C in MES pH 5.0 using 10 mM of substrate.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 6.00
    Constituents: 0.39% MES, 0.002% PMSF, 0.06% EDTA, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Acid phosphatase 1 soluble
    • Acid phosphatase of erythrocyte
    • ACP1
    • Adipocyte acid phosphatase
    • Cytoplasmic phosphotyrosyl protein phosphatase
    • HAAP
    • LMW-PTP
    • LMW-PTPase
    • Low molecular weight cytosolic acid phosphatase
    • Low molecular weight phosphotyrosine protein phosphatase
    • PAP1
    • PAP2
    • phosphatase, acid, of erythrocyte
    • PPAC_HUMAN
    • Protein tyrosine phosphatase
    • PTPase
    • Purple acid phosphatase
    • Red cell acid phosphatase 1
    • testicular secretory protein Li 37
    see all
  • Function
    Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
  • Tissue specificity
    T-lymphocytes express only isoform 2.
  • Sequence similarities
    Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3 µg ab168008.

References

ab168008 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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