Recombinant human Acid phosphatase/ACP1 protein (ab87493)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human Acid phosphatase/ACP1 protein -
Biological activity
Specific activity: > 60,000 unit/mg of protein. Unit definition: One unit is defined as the amount of enzyme that will hydrolyze 1nmole of p-nitrophenyl phosphate per minute at 37oC in MES pH5.0 using 10mM of substrate.
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Purity
> 95 % SDS-PAGE.
ab87493 is purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab87493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).This product was previously labelled as Acid Phosphatase
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 6.00
Constituents: 0.39% MES, 0.00174% PMSF, 0.0584% EDTA, 10% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Acid phosphatase 1 soluble
- Acid phosphatase of erythrocyte
- ACP1
see all -
Function
Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity. -
Tissue specificity
T-lymphocytes express only isoform 2. -
Sequence similarities
Belongs to the low molecular weight phosphotyrosine protein phosphatase family. -
Cellular localization
Cytoplasm. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab87493 has not yet been referenced specifically in any publications.