Recombinant Human Actin Regulatory Protein CAPG/MCP (ab95385)

Description

  • Product name

    Recombinant Human Actin Regulatory Protein CAPG/MCP
    See all Actin Regulatory Protein CAPG/MCP proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    ab95385 is purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MYTAIPQSGS PFPGSVQDPG LHVWRVEKLK PVPVAQENQG VFFSGDSYLV LHNGPEEVSH LHLWIGQQSS RDEQGACAVL AVHLNTLLGE RPVQHREVQG NESDLFMSYF PRGLKYQEGG VESAFHKTST GAPAAIKKLY QVKGKKNIRA TERALNWDSF NTGDCFILDL GQNIFAWCGG KSNILERNKA RDLALAIRDS ERQGKAQVEI VTDGEEPAEM IQVLGPKPAL KEGNPEEDLT ADKANAQAAA LYKVSDATGQ MNLTKVADSS PFALELLISD DCFVLDNGLC GKIYIWKGRK ANEKERQAAL QVAEGFISRM QYAPNTQVEI LPQGRESPIF KQFFKDWK

Specifications

Our Abpromise guarantee covers the use of ab95385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

     This product was previously labelled as Actin Regulatory Protein CAPG

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol

General Info

  • Alternative names

    • Actin capping protein GCAP39
    • Actin regulatory protein CAP G
    • Actin regulatory protein CAP-G
    • AFCP
    • Capg
    • CAPG_HUMAN
    • Capping actin protein gelsolin like
    • Capping protein (actin filament) gelsolin like
    • Capping protein gelsolin like
    • Epididymis secretory protein Li 66
    • gCap39
    • Gelsolin like capping protein
    • HEL S 66
    • Macrophage capping protein
    • Macrophage-capping protein
    • mbh1
    • MCP
    • Myc basic motif homolog 1
    see all
  • Function

    Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
  • Tissue specificity

    Macrophages and macrophage-like cells.
  • Sequence similarities

    Belongs to the villin/gelsolin family.
    Contains 3 gelsolin-like repeats.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3µg ab95385.

References

ab95385 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for kindly confirming these details. I am sorry this vial of protein has not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab95385 has been added to your order number PO-15699 - DG. This has Abcam order reference 1175608. This has a different lot number.

I would like to reassure you that this free of charge replacement vial is also covered by our Abpromise guarantee. Should the customer still be experiencing difficulties with the new vial, or if you have any further questions, please do not hesitate to let me know.

Thank you for your help and cooperation with this case. Please do not hesitate to contact me if you need anything further.

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Answer

Thank you for your message and for kindly providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a free of charge replacement or credit note in compensation.

I look forward to hearing from you with details of how you would like to proceed.

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Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab95385 Actin Regulatory Protein CAPG (Human)is tested and covered by our 6 month guarantee for use inSDS gels. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

Reviewing this case, I would like to investigate this further for you and obtain some further information for quality monitoring. In order to do so, I would also appreciate if you can confirm some further details:

1. Please provide information on the primary and secondary antibodies that has been used to detect this protein in western blot. Were these the samethat were used to detectthe first successful 2 batches of protein? Are the current vials of antibodies working well with other samples?

2. I can recommend to run an SDS gel andassess the quality of the protein sample using coomassie blue, to ensure it has not degraded. Could you confirm if this has been tried already? What were the results?

3. I would appreciate if you are able to provide any images, which would help us to assess the results (from the batches that have worked, and the ones that have not).

I hope this information is helpful, thank you for your cooperation.Please do not hesitate to contact me again with the further requested details and I hope we can resolve this case for you as soonas possible.

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