Description

  • Product name

    Recombinant human active Caspase-2 protein
  • Biological activity

    SPECIFIC ACTIVITY: 10,000 – 13,000 units/mg UNIT DEFINATION: One unit of the recombinant caspase-2 is the enzyme activity that cleaves 1 nmol of the caspase substrate VDVAD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab52098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Additional notes

    The lyophilized caspase-2 is stable for 1 year at –70oC.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Constituents: PBS, 15% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute to 1 unit per µl in PBS containing 15% glycerol.

General Info

  • Alternative names

    • CASP2
    • Caspase 2
    • ICH 1L
    • ICH1
    • ICH1 protease
    • ICH1L
    • NEDD 2 apoptosis regulatory gene
    • NEDD2
    see all
  • Relevance

    Caspase 2 is involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. During apoptosis, procaspase-2 is processed at aspartate residues. The processed form of caspase-2 consists of large (19 kDa) and small (12 kDa) subunits which associate to form the active enzyme.
  • Cellular localization

    Cytosol, membrane

Images

References

ab52098 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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