Recombinant Human ACY-1 protein (His tag) (ab203546)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% Densitometry
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: MS, SDS-PAGE, WB, ELISA
Description
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Product name
Recombinant Human ACY-1 protein (His tag)
See all ACY-1 proteins and peptides -
Purity
> 95 % Densitometry. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MTSKGPEEEH PSVTLFRQYL RIRTVQPKPD YGAAVAFFEE TARQLGLGCQ KVEVAPGYVV TVLTWPGTNP TLSSILLNSH TDVVPVFKEH WSHDPFEAFK DSEGYIYARG AQDMKCVSIQ YLEAVRRLKV EGHRFPRTIH MTFVPDEEVG GHQGMELFVQ RPEFHALRAG FALDEGIANP TDAFTVFYSE RSPWWVRVTS TGRPGHASRF MEDTAAEKLH KVVNSILAFR EKEWQRLQSN PHLKEGSVTS VNLTKLEGGV AYNVIPATMS ASFDFRVAPD VDFKAFEEQL QSWCQAAGEG VTLEFAQKWM HPQVTPTDDS NPWWAAFSRV CKDMNLTLEP EIMPAATDNR YIRAVGVPAL GFSPMNRTPV LLHDHDERLH EAVFLRGVDI YTRLLPALAS VPALPSDSLE HHHHHH -
Predicted molecular weight
47 kDa including tags -
Amino acids
1 to 408 -
Tags
His tag C-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab203546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
Western blot
ELISA
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Mass spectrometry
LC-MS/MS -
Form
Lyophilized -
Additional notes
Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 100% PBS
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ReconstitutionAdd 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
General Info
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Alternative names
- ACY 1
- ACY-1
- Acy1
see all -
Function
Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). -
Tissue specificity
Expression is highest in kidney, strong in brain and weaker in placenta and spleen. -
Involvement in disease
Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D) [MIM:609924]. ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids. -
Sequence similarities
Belongs to the peptidase M20A family. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human ACY-1 protein (His tag) (ab203546)
14% SDS-PAGE analysis of ab203546.
Lane 1: M.W. marker – 14, 21, 31, 45, 66, 97 kDa
Lane 2: Reduced and heated sample, 2.5μg / lane
Lane 3: Non-reduced and non-heated sample, 2.5μg / lane
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab203546 has not yet been referenced specifically in any publications.
