Recombinant Human Acyl-coenzyme A Thioesterase 8 protein (ab156739)

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SDS-PAGE - Recombinant Human Acyl-coenzyme A Thioesterase 8 protein (ab156739)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human Acyl-coenzyme A Thioesterase 8 protein

    • Purity

      > 95 % SDS-PAGE.
      ab156739 was purified by using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      O14734

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSMSSPQAP EDGQGCGDRG DPPGDLRSVL VTTVLNLEPL DEDLFRGRHY WVPAKRLFGG QIVGQALVAA AKSVSEDVHV HSLHCYFVRA GDPKLPVLYQ VERTRTGSSF SVRSVKAVQH GKPIFICQAS FQQAQPSPMQ HQFSMPTVPP PEELLDCETL IDQYLRDPNL QKRYPLALNR IAAQEVPIEI KPVNPSPLSQ LQRMEPKQMF WVRARGYIGE GDMKMHCCVA AYISDYAFLG TALLPHQWQH KVHFMVSLDH SMWFHAPFRA DHWMLYECES PWAGGSRGLV HGRLWRQDGV LAVTCAQEGV IRVKPQVSES KL

      • Predicted molecular weight

        38 kDa including tags

      • Amino acids

        1 to 319

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab156739 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.03% DTT, 0.32% Tris HCl, 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride

    General Info

    • Alternative names

      • ACTEIII
      • Acot8
      • ACOT8_HUMAN
      • Acyl CoA thioesterase 8
      • Acyl-CoA thioesterase 8
      • Acyl-coenzyme A thioesterase 8
      • Choloyl coenzyme A thioesterase
      • Choloyl-coenzyme A thioesterase
      • hACTE III
      • hACTE-III
      • hACTEIII
      • HIV Nef associated acyl CoA thioesterase
      • HIV-Nef-associated acyl-CoA thioesterase
      • HNAACTE
      • hTE
      • Long chain fatty acyl CoA hydrolase
      • Palmitoyl CoA hydrolase
      • Peroxisomal acyl CoA thioesterase 1
      • Peroxisomal acyl coenzyme A thioester hydrolase 1
      • Peroxisomal acyl-coenzyme A thioester hydrolase 1
      • Peroxisomal long chain acyl CoA thioesterase 1
      • Peroxisomal long-chain acyl-CoA thioesterase 1
      • PTE 1
      • PTE 2
      • PTE-1
      • PTE-2
      • PTE1
      • PTE2
      • Thioesterase II
      see all

    • Function

      Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (By similarity). May be involved in the metabolic regulation of peroxisome proliferation.

    • Tissue specificity

      Detected in a T-cell line (at protein level). Ubiquitous.

    • Sequence similarities

      Belongs to the C/M/P thioester hydrolase family.

    • Cellular localization

      Peroxisome.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Acyl-coenzyme A Thioesterase 8 protein (ab156739)
      SDS-PAGE - Recombinant Human Acyl-coenzyme A Thioesterase 8 protein (ab156739)
      15% SDS-PAGE analysis of ab156739 (3µg)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab156739? Please let us know so that we can cite the reference in this datasheet.

    ab156739 has not yet been referenced specifically in any publications.

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