Recombinant Human MS2 protein - BSA and Azide free (ab174041)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Description
-
Product name
Recombinant Human MS2 protein - BSA and Azide free -
Purity
> 90 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Carrier free
Yes -
Nature
Recombinant -
-
Species
Human -
Sequence
IAPSRPWALMEQYEVVLPWRLPGPRVRRALPSHLGLHPERVSYVLGATGH NFTLHLRKNRDLLGSGYTETYTAANGSEVTEQPRGQDHCFYQGHVEGYPD SAASLSTCAGLRGFFQVGSDLHLIEPLDEGGEGGRHAVYQAEHLLQTAGT CGVSDDSLGSLLGPRTAAVFRPRPGDSLPSRETRYVELYVVVDNAEFQML GSEAAVRHRVLEVVNHVDKLYQKLNFRVVLVGLEIWNSQDRFHVSPDPSV TLENLLTWQARQRTRRHLHDNVQLITGVDFTGTTVGFARVSAMCSHSSGA VNQDHSKNPVGVACTMAHEMGHNLGMDHDENVQGCRCQERFEAGRCIMAG SIGSSFPRMFSDCSQAYLESFLERPQSVCLANAPDLSHLVGGPVCGNLFV ERGEQCDCGPPEDCRNRCCNSTTCQLAEGAQCAHGTCCQECKVKPAGELC RPKKDMCDLEEFCDGRHPECPEDAFQENGTP -
Predicted molecular weight
72 kDa including tags -
Molecular weight information
This protein has a calculated MW of 53.8 kDa, which migrates as a 130-150 kDa band in DTT-reduced SDS-PAGE due to glycosylation. Two main fragments bands are visible with apparent MW of 58-68 kDa and 35-40 kDa respectively in reduced SDS-PAGE as a result of its being prone to proteolytic cleavage. -
Amino acids
17 to 497 -
Tags
His tag C-Terminus
-
-
Description
Recombinant Human MS2 protein (BSA and azide free)
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab174041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Form
Lyophilized -
Additional notes
Lyophilized from 0.22 μm filtered solution.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.4
Constituents: 0.88% Sodium chloride, 0.61% Tris, 0.11% Calcium chloride, 5% Trehalose -
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
General Info
-
Alternative names
- A Disintegrin And Metalloproteinase domain 8
- ADAM 8
- ADAM 8 precursor
see all -
Function
Possible involvement in extravasation of leukocytes. -
Tissue specificity
Expressed on neutrophils and monocytes. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
Cellular localization
Membrane. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab174041 has not yet been referenced specifically in any publications.