Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB, ELISA
Product nameRecombinant Human ADAMTS4 protein
Expression systemWheat germ
Protein lengthProtein fragment
SequenceMSQTGSHPGRGLAGRWLWGAQPCLLLPIVPLSWLVWLLLLLLASLLPSAR LASPLPREEEIVFPEKLNGSVLPGSGAPARLLCRLQAFGETLLLELEQDS GVQVEGLTVQYLGQAPELLGGAEPGTYLTGTINGDPESVASLHWDGGALL GVLQYRGAELHLQPLEGGTPNSAGGPGAHILRRKSPASGQGPMCNVKAPL GSPSPRPRRAKRFASLSRFVETLVVADDKMAAFHGAGLKRYLLTVMAAAA KAFKHPSIRNPVSLVVTRLVILGSGEEGPQVGPSAAQTLRSFCAWQRGLN TPEDSDPDHFDTAILFTRQVRPQSAPQAMHCTILRSATT
Predicted molecular weight62 kDa including tags
Amino acids1 to 339
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab132081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.79% Tris HCl
- A disintegrin and metalloproteinase with thrombospondin motifs 4
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 4
FunctionCleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
Tissue specificityExpressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 TSP type-1 domain.
DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab132081 has not yet been referenced specifically in any publications.