Recombinant Human Adiponectin protein (ab13882)

Submit a review Q&A (4)References (1)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      Q15848
    • Species
      Human
    • Sequence
      MRGSHHHHHH GSGHDQETTT QGPGVLLPLP KGACTGWMAG IPGHPGHNGA PGRDGRDGTP GEKGEKGDPG LIGPKGDIGE TGVPGAEGPR GFPGIQGRKG EPGEGAYVYR SAFSVGLETY
    • Amino acids
      15 to 244
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab13882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
  • Form
    Lyophilised
  • Additional notes


    The Human Adiponectin is created as a recombinant protein with N-terminal fusion of HisTag. The Human Adiponectin His-Tagged Fusion Protein, produced in E. coli, is 26.4 kDa protein containing 230 amino acid residues of the human Adiponectin and 12 additional amino acid residues - HisTag

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.02% Tris buffered saline, 0.87% Sodium chloride

  • Reconstitution
    Store lyophilized protein at -20°C. Add 0.2 ml of distilled water and let the lyophilized pellet dissolve completely.

General Info

  • Alternative names
    • 30 kDa adipocyte complement related protein
    • 30 kDa adipocyte complement-related protein
    • ACDC
    • ACRP30
    • ADIPO_HUMAN
    • Adipocyte
    • Adipocyte C1q and collagen domain containing protein
    • Adipocyte complement related 30 kDa protein
    • Adipocyte complement related protein of 30 kDa
    • Adipocyte complement-related 30 kDa protein
    • adipocyte-specific secretory protein
    • Adiponectin
    • Adiponectin precursor
    • adiponectin, C1Q and collagen domain containing
    • Adipoq
    • Adipose most abundant gene transcript 1
    • Adipose most abundant gene transcript 1 protein
    • Adipose specific collagen like factor
    • ADIPQTL1
    • ADPN
    • APM 1
    • apM-1
    • APM1
    • C1q and collagen domain-containing protein
    • GBP28
    • Gelatin binding protein
    • Gelatin binding protein 28
    • Gelatin-binding protein
    see all
  • Function
    Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
  • Tissue specificity
    Synthesized exclusively by adipocytes and secreted into plasma.
  • Involvement in disease
    Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
    Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • Sequence similarities
    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Domain
    The C1q domain is commonly called the globular domain.
  • Post-translational
    modifications
    Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
    HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
    O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

This product has been referenced in:
  • Kabara E  et al. Adiponectin links adipose tissue function and monocyte inflammatory responses during bovine metabolic stress. Comp Immunol Microbiol Infect Dis 37:49-58 (2014). Read more (PubMed: 24296305) »
See 1 Publication for this product

Publishing research using ab13882? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

I've submitted my abreview of the product. Is that allI have to do for the discount to be applied?
Sincerely,

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Answer

Thank you for your reply.
My colleague is out of the office but I will be happy to help you with this enquiry.
Yes, submitting the Abreview is all that you need to do in order to active the discount. I have added the discount code to our internal notes section of the Abreview for ab62551, so just include this discount code with your next order to Abcam, and you will receive a 100% discount off of a primary antibody.
I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you.

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Question

I would like to test ab22554 and ab13882 for use in WB on cow samples.

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Answer

For ab13882 DISCOUNT CODE: ***(valid on proteins)
For ab22554 DISCOUNT CODE: *** (valid on primary antibodies)
Expiration date: July 28th, 2012
I am very pleased to hear you would like to accept our offer and test ab13882 and ab22554 for use in WB on cow samples. Each code will give you 1 free unit of the same product type before the expiration date. To redeem this offer, please submit an Abreview for each product and include the matching code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

Detection of the protein ab13882 (5ug/well) gave no signal with a His detection kit which has the ability to detect levels as low as 10ng. A positive control of tag marked protein gave good signal.

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Answer

I wanted to let you know that the outcome of my investigation with the source of ab13882 was that it was found that the lot you had received has not given satisfactory results in the recent tests. We have immediately removed all the stock of this product and are contacting all customers who purchased this lot to offer a replacement of the new lot. I hope this unfortunate event will not deter you from purchasing from Abcam in the future and wish you all the best with your research,

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Question

I´ll answer the questions below. But I´m afraid we misunderstood each other. I ordered recombinant adiponectin (ab13882-100 lot 71252)and used it as standard in our ELISA. The lyophilized adiponectin was reconstituted as described in the product sheet: 0.1 M Acetat buffer pH 4 and after reconstitution we immediately diluted the adiponectin to a concentration of 10µg/ml in PBS to neutral pH. Than we used the diluted adiponectin as standard material in our ELISA, which functions very well with recombinant adiponectin of other providers. So there is no problem with the ELISA itself, but with your adiponectin. What do you think about reconstitution procedure - it seems to me rather rough, is adiponectin not solvable in neutral PBS? I would like to try this way, if you can provide another aliquot of adiponectin. 1. Please describe the problem (high background, no signal, non-specific colour development, poor standard curve etc). your recombinant adiponectin gives no signal - adiponectin of other sources does! 2. What type of ELISA are you performing? (Direct ELISA, indirect ELISA, sandwich ELISA etc.) sandwich elisa 7. Which detection system did you use? Substrate? Streptavidin-POD and TMB 8. Did you apply positive and negative controls along with the samples? Please specify. What did you use for standards? yes and our positive human serum controls also work very well!

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Answer

Thank you for your reply. First of all, please ignore the invoice until we sort this problem out. I have looked through the details you have forwarded to us and unfortunately from point 3. to 6. on the ELISA Questionnaire I can't find the answers. Although I understand that your ELISA assay work nicely with your positive control, the extra information regarding samples, blocking, dilution of the primary antibody etc would have been very important for us to be able to identify the source of the problem. You have carried out a Sandwich type of ELISA. Have you actually coated the wells with the primary antibody first or with the samples and/or the recombinant protein? How much recombinant protein have you used? In what concentration range have you tested the protein? I would also like to know the primary antibody you used. Is it monoclonal or polyclonal antibody? We look forward to hearing from you soon.

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