Recombinant human ADK protein (Active) (ab227392)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: MS, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human ADK protein (Active)
See all ADK proteins and peptides -
Biological activity
Specific activity is > 30 pmol/min/µg and is defined as the amount of enzyme that converts 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37°C in a couple system with PK and LDH.
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Purity
> 95 % SDS-PAGE.
ab227392 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMRENILFGMGNPLLDISAVVDKDFLDKYSL KPNDQILAEDKHKELFDELVKKFKVEYHAGGSTQNSIKVAQWMIQQPHKA ATFFGCIGIDKFGEILKRKAAEAHVDAHYYEQNEQPTGTCAACITGDNRS LIANLAAANCYKKEKHLDLEKNWMLVEKARVCYIAGFFLTVSPESVLKVA HHASENNRIFTLNLSAPFISQFYKESLMKVMPYVDILFGNETEAATFARE QGFETKDIKEIAKKTQALPKMNSKRQRIVIFTQGRDDTIMATESEVTAFA VLDQDQKEIIDTNGAGDAFVGGFLSQLVSDKPLTECIRAGHYAASIIIRR TGCTFPEKPDFH -
Predicted molecular weight
41 kDa including tags -
Amino acids
22 to 362 -
Tags
His tag N-Terminus -
Additional sequence information
NP_006712.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab227392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
Functional Studies
SDS-PAGE
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 0.03% EDTA, 20% Glycerol (glycerin, glycerine), 0.29% Sodium chloride, 0.32% Tris HClThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 2310026J05Rik
- 5033405D03Rik
- Adenosine 5''-phosphotransferase
see all -
Function
ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. -
Tissue specificity
Widely expressed. Highest level in placenta, liver, muscle and kidney. -
Pathway
Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1. -
Sequence similarities
Belongs to the carbohydrate kinase pfkB family. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227392 has not yet been referenced specifically in any publications.