Key features and details
- Expression system: Wheat germ
- Suitable for: WB, SDS-PAGE, ELISA
Product nameRecombinant Human AGO3 protein
Expression systemWheat germ
Protein lengthFull length protein
SequenceMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCN VTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQE QKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLV RSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHG VWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGM PIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVK RVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQR PSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQR PRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYE LLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAG TTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLT YQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQ SNGRDPQALAKAVQIHQDTLRTMYFA
Predicted molecular weight98 kDa including tags
Amino acids1 to 626
Our Abpromise guarantee covers the use of ab131912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as Argonaute 3 / eIF2C 3.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.3% Glutathione, 0.79% Tris HCl
- Ago 3
FunctionRequired for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs.
Sequence similaritiesBelongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
Cellular localizationCytoplasm > P-body.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab131912 has not yet been referenced specifically in any publications.