Recombinant human AK 1 protein (ab167983)

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SDS-PAGE - Recombinant human AK 1 protein (ab167983)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Active: Yes
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, Functional Studies

    Description

    • Product name

      Recombinant human AK 1 protein
      See all AK 1 proteins and peptides

    • Biological activity

      Specific activity: > 6.0 units/ml. One unit will convert 2.0 µmoles of ADP to ATP + AMP per minute at pH 7.5 at 25C.

    • Purity

      > 95 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      P00568

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMEEKLKKTKIIFVVGGPGSGKGTQCEKIVQ KYGYTHLSTGDLLRSEVSSGSARGKKLSEIMEKGQLVPLETVLDMLRDAM VAKVNTSKGFLIDGYPREVQQGEEFERRIGQPTLLLYVDAGPETMTQRLL KRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVD SVFSQVCTHLDALK

      • Predicted molecular weight

        24 kDa including tags

      • Amino acids

        1 to 194

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab167983 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Liquid

    • Additional notes

      Previously labelled as Adenylate Kinase 1. 

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.32% Tris HCl, 10% Glycerol

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • Adenylate kinase 1
      • Adenylate kinase isoenzyme 1
      • Adenylate kinase soluble
      • AK 1
      • Ak1
      • ATP AMP transphosphorylase
      • ATP-AMP transphosphorylase 1
      • KAD1
      • KAD1_HUMAN
      • Myokinase
      see all

    • Function

      Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.

    • Involvement in disease

      Defects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency [MIM:612631].

    • Sequence similarities

      Belongs to the adenylate kinase family.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human AK 1 protein (ab167983)
      SDS-PAGE - Recombinant human AK 1 protein (ab167983)
      15% SDS PAGE analysis of ab167983 at 3 µg.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab167983? Please let us know so that we can cite the reference in this datasheet.

    ab167983 has not yet been referenced specifically in any publications.

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