Recombinant human AKR1B10 protein (ab168014)

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SDS-PAGE - Recombinant human AKR1B10 protein (ab168014)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Active: Yes
    • Suitable for: SDS-PAGE, Functional Studies

    Description

    • Product name

      Recombinant human AKR1B10 protein
      See all AKR1B10 proteins and peptides

    • Biological activity

      The specific activity of AKR1B10 with 5 mM glyceraldehyde is approximately ~100 mU/mg. (Specific activity is calculated by the decrease of absorption at 340 nm in presence of 2-10 µg AKR1B10, 5mM glyceraldehyde, 200 µM NADPH in 0.1 M sodium phosphate buffer, pH 7.5).

    • Purity

      > 90 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      O60218

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY

      • Predicted molecular weight

        36 kDa

      • Amino acids

        1 to 316

    Specifications

    Our Abpromise guarantee covers the use of ab168014 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Liquid

    • Additional notes

      Centrifuge the vial prior to opening
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: Tris HCl, 50% Glycerol

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • AK1BA_HUMAN
      • AKR1B10
      • AKR1B11
      • AKR1B12
      • Aldo keto reductase family 1 member B10
      • aldo keto reductase family 1 member B11
      • Aldo-keto reductase family 1 member B10
      • Aldo-keto reductase family 1 member B10 (aldose reductase)
      • Aldo-keto reductase family 1 member B11 (aldose reductase-like)
      • Aldose reductase like
      • aldose reductase like 1
      • aldose reductase like peptide
      • Aldose reductase related protein
      • Aldose reductase-like
      • Aldose reductase-related protein
      • ALDRLn
      • ARL 1
      • ARL-1
      • ARL1
      • ARL1 Human small intestine reductase
      • ARP
      • hARP
      • HIS
      • HSI
      • SI reductase
      • Small intestine reductase
      see all

    • Function

      Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.

    • Tissue specificity

      Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.

    • Sequence similarities

      Belongs to the aldo/keto reductase family.

    • Cellular localization

      Lysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human AKR1B10 protein (ab168014)
      SDS-PAGE - Recombinant human AKR1B10 protein (ab168014)
      15% SDS-PAGE analysis of 3 µg ab168014.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab168014? Please let us know so that we can cite the reference in this datasheet.

    ab168014 has not yet been referenced specifically in any publications.

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