Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY
    • Molecular weight
      39 kDa including tags
    • Amino acids
      1 to 323
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab168012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity is approximately 0.15 – 0.2 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C. Specific activity was expressed as units/mg protein.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    >90% by SDS-PAGE.

  • Form
    Liquid
  • Additional notes
    Centrifuge the vial prior to opening
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 2-dihydrobenzene-1
    • 2-diol dehydrogenase
    • 20 alpha (3 alpha) hydroxysteroid dehydrogenase
    • 20 ALPHA HSD
    • 20 alpha hydroxysteroid dehydrogenase
    • 20-alpha-HSD
    • 20-alpha-hydroxysteroid dehydrogenase
    • 20ALPHAHSD
    • 2ALPHAHSD
    • AK1C1
    • AK1C1_HUMAN
    • AKR1C1
    • Aldo keto reductase family 1 member C1
    • aldo-keto reductase C
    • Aldo-keto reductase family 1 member C1
    • Aldo-keto reductase family, 1 member 1
    • C9
    • Chlordecone reductase homolog
    • Chlordecone reductase homolog HAKRC
    • DD1
    • DD1/DD2
    • DDH
    • DDH1
    • Dihydrodiol dehydrogenase 1
    • Dihydrodiol dehydrogenase 1/2
    • dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase
    • dihydrodiol dehydrogenase isoform DD1
    • Dihydrodiol dehydrogenase, type 1
    • H37
    • HAKRC
    • HBAB
    • Hepatic dihydrodiol dehydrogenase
    • High affinity hepatic bile acid-binding protein
    • High-affinity hepatic bile acid-binding protein
    • Indanol dehydrogenase
    • MBAB
    • MGC8954
    • Trans-1
    • Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase
    • Type II 3 alpha hydroxysteroid dehydrogenase
    see all
  • Function
    Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation.
  • Tissue specificity
    Expressed in all tissues tested including liver, prostate, testis, adrenal gland, brain, uterus, mammary gland and keratinocytes. Highest levels found in liver, mammary gland and brain.
  • Sequence similarities
    Belongs to the aldo/keto reductase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3 µg ab168012.

References

ab168012 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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