Description

  • Product name

    Recombinant human AKT2 protein
    See all AKT2 proteins and peptides
  • Purity

    > 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab60322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab204851 (AKT peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Akt2
    • AKT2_HUMAN
    • HIHGHH
    • Murine thymoma viral (v-akt) homolog 2
    • murine thymoma viral (v-akt) homolog-2
    • Oncogene AKT2 protein kinase B beta
    • PKB
    • PKB beta
    • PKBB
    • PKBBETA
    • PRKBB
    • Protein kinase Akt 2
    • Protein kinase Akt-2
    • Protein kinase B beta
    • RAC beta
    • rac protein kinase beta
    • RAC-BETA
    • RAC-beta serine/threonine-protein kinase
    • RAC-PK-beta
    • RACbeta
    • v akt murine thymoma viral oncogene homolog 2
    • V-AKT murine thymoma viral oncogene homolog 2
    see all
  • Function

    General protein kinase capable of phosphorylating several known proteins.
  • Tissue specificity

    Expressed in all human cell types so far analyzed.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylation on Thr-309 and Ser-474 is required for full activity.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Information by UniProt

Images

  • The specific activity of AKT2 (ab60322) was determined to be 80 nmol/min/mg as per activity assay protocol and was equivalent to 52 nmol/min/mg as per radiometric assay
  • SDS PAGE analysis of ab60322
  • SDS PAGE analysis of ab60322
  • Sample Kinase Activity Plot.
  • ab60322 on SDS-PAGE, MW ~85kDa.

References

ab60322 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab60322.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up