Bioactive grade

Recombinant human ALDH2 protein (Active) (ab87415)

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SDS-PAGE - Recombinant human ALDH2 protein (Active) (ab87415)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/mg
    • Active: Yes
    • Suitable for: SDS-PAGE, Functional Studies, WB

    Description

    • Product name

      Recombinant human ALDH2 protein (Active)
      See all ALDH2 proteins and peptides

    • Biological activity

      > 250pmol/min/ug

      Obtained by measuring the increase of NADH in absorbance at 340 nm resulting from the reduction of NAD at pH 8.0 at 25C.

       

       

    • Purity

      > 90 % SDS-PAGE.
      ab87415 is purified using conventional chromatography techniques.

    • Endotoxin level

      < 1.000 Eu/mg

    • Expression system

      Escherichia coli

    • Accession

      P05091

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S

      • Predicted molecular weight

        55 kDa

      • Amino acids

        18 to 517

      • Additional sequence information

        (NP_000681)

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab87415 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

      Western blot

    • Form

      Liquid

    • Additional notes

      MW confirmed by MALDI-TOF.

      Concentration determined by Absorbance at 280 nm.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.50
      Constituents: 0.0154% DTT, 0.316% Tris HCl, 0.0292% EDTA, 10% Glycerol (glycerin, glycerine)

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • Acetaldehyde dehydrogenase 2
      • Aldehyde dehydrogenase 2 family
      • Aldehyde dehydrogenase 2 family (mitochondrial)
      • Aldehyde dehydrogenase mitochondrial
      • Aldehyde dehydrogenase, mitochondrial
      • ALDH 2
      • ALDH class 2
      • ALDH E2
      • ALDH-E2
      • Aldh2
      • ALDH2_HUMAN
      • ALDHI
      • ALDM
      • Liver mitochondrial ALDH
      • MGC1806
      • Mitochondrial aldehyde dehydrogenase 2
      • MS767
      • Nucleus encoded mitochondrial aldehyde dehydrogenase 2
      see all

    • Pathway

      Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.

    • Sequence similarities

      Belongs to the aldehyde dehydrogenase family.

    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human ALDH2 protein (Active) (ab87415)
      SDS-PAGE - Recombinant human ALDH2 protein (Active) (ab87415)

      SDS-PAGE analysis of ab87415 (3 µg) under reducing conditions and visualized by coomassie blue stain.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab87415? Please let us know so that we can cite the reference in this datasheet.

    ab87415 has been referenced in 1 publication.

    • Yasgar A  et al. A High-Content Assay Enables the Automated Screening and Identification of Small Molecules with Specific ALDH1A1-Inhibitory Activity. PLoS One 12:e0170937 (2017). PubMed: 28129349

    Customer reviews and Q&As

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    Question

    I do not understand the meaning of "specific activity is < 1 units/ml".
    When we use it, we need assess the activity again. As we lack of
    experience of ALDH activity assay, could you let us know the protocol you
    used for the assessment? Thanks again for the help in advance.

    Read More
    Answer

    Thank you for your reply. The specific information activity for these products is as follows:


    ab87415: Specific activity is > 0.14 units/ml

    ab93463 and ab117154: Specific activity is > 0.5 units/ml

    The protocol used to measure the specific activity is:

    Activity Assay

    1) Prepare a 3ml reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA.
    2) Equilibrate to 25C and monitor the A340nm until value is constant using a spectrophotometer.
    3) Add 5ug of recombinant ALDH2 into reaction mixture and mix immediately.
    4) Record the increase in A340nm for 5 minutes



    I hope this helps, please let me know if you need any additional information.

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