Recombinant human ALDH2 protein (Active) (ab87415)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      P05091
    • Species
      Human
    • Sequence
      MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S
    • Molecular weight
      55 kDa
    • Amino acids
      18 to 517
    • Additional sequence information
      (NP_000681)

Specifications

Our Abpromise guarantee covers the use of ab87415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    > 250pmol/min/ug

    Obtained by measuring the increase of NADH in absorbance at 340 nm resulting from the reduction of NAD at pH 8.0 at 25C.

     

     

  • Applications

    SDS-PAGE

    Functional Studies

    Western blot

  • Endotoxin level
    < 1.000 Eu/mg
  • Purity
    > 90 % SDS-PAGE.
    ab87415 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Additional notes

    MW confirmed by MALDI-TOF.

    Concentration determined by Absorbance at 280 nm.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 0.0292% EDTA, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Acetaldehyde dehydrogenase 2
    • Aldehyde dehydrogenase 2 family
    • Aldehyde dehydrogenase 2 family (mitochondrial)
    • Aldehyde dehydrogenase mitochondrial
    • Aldehyde dehydrogenase, mitochondrial
    • ALDH 2
    • ALDH class 2
    • ALDH E2
    • ALDH-E2
    • Aldh2
    • ALDH2_HUMAN
    • ALDHI
    • ALDM
    • Liver mitochondrial ALDH
    • MGC1806
    • Mitochondrial aldehyde dehydrogenase 2
    • MS767
    • Nucleus encoded mitochondrial aldehyde dehydrogenase 2
    see all
  • Pathway
    Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
  • Sequence similarities
    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human ALDH2 protein (ab87415)
    SDS-PAGE - Recombinant human ALDH2 protein (ab87415)

    3µg of ab87415 on 15% SDS-PAGE, stained with Coomassie Blue.

References

This product has been referenced in:
  • Yasgar A  et al. A High-Content Assay Enables the Automated Screening and Identification of Small Molecules with Specific ALDH1A1-Inhibitory Activity. PLoS One 12:e0170937 (2017). Read more (PubMed: 28129349) »
See 1 Publication for this product

Publishing research using ab87415? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I do not understand the meaning of "specific activity is < 1 units/ml".
When we use it, we need assess the activity again. As we lack of
experience of ALDH activity assay, could you let us know the protocol you
used for the assessment? Thanks again for the help in advance.

Read More
Answer

Thank you for your reply. The specific information activity for these products is as follows:


ab87415: Specific activity is > 0.14 units/ml

ab93463 and ab117154: Specific activity is > 0.5 units/ml

The protocol used to measure the specific activity is:

Activity Assay

1) Prepare a 3ml reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA.
2) Equilibrate to 25C and monitor the A340nm until value is constant using a spectrophotometer.
3) Add 5ug of recombinant ALDH2 into reaction mixture and mix immediately.
4) Record the increase in A340nm for 5 minutes



I hope this helps, please let me know if you need any additional information.

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