Description

  • Product name

    Recombinant human ALDH3A1 protein
    See all ALDH3A1 proteins and peptides
  • Biological activity

    Specific activity is >5 units/ml and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 umole of acetaldehyde to acetic acid per minute at pH 8.0 at 25C in the presence of beta-NAD, potassium and thiols.
  • Purity

    > 95 % SDS-PAGE.
    ab93463 is purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MSKISEAVKR ARAAFSSGRT RPLQFRIQQL EALQRLIQEQ EQELVGALAA DLHKNEWNAY YEEVVYVLEE IEYMIQKLPE WAADEPVEKT PQTQQDELYI HSEPLGVVLV IGTWNYPFNL TIQPMVGAIA AGNAVVLKPS ELSENMASLL ATIIPQYLDK DLYPVINGGV PETTELLKER FDHILYTGST GVGKIIMTAA AKHLTPVTLE LGGKSPCYVD KNCDLDVACR RIAWGKFMNS GQTCVAPDYI LCDPSIQNQI VEKLKKSLKE FYGEDAKKSR DYGRIISARH FQRVMGLIEG QKVAYGGTGD AATRYIAPTI LTDVDPQSPV MQEEIFGPVL PIVCVRSLEE AIQFINQREK PLALYMFSSN DKVIKKMIAE TSSGGVAAND VIVHITLHSL PFGGVGNSGM GSYHGKKSFE TFSHRRSCLV RPLMNDEGLK VRYPPSPAKM TQH

Specifications

Our Abpromise guarantee covers the use of ab93463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.316% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Acetaldehyde dehydrogenase 3
    • AHD 4
    • AHD C
    • AHD4
    • AHDC
    • AL3A1_HUMAN
    • Aldehyde dehydrogenase
    • Aldehyde dehydrogenase 3
    • Aldehyde dehydrogenase 3 family member A1
    • Aldehyde dehydrogenase 3A1
    • Aldehyde dehydrogenase class 3
    • Aldehyde dehydrogenase dimeric NADP preferring
    • Aldehyde dehydrogenase family 3 member A1
    • Aldehyde dehydrogenase family 3 subfamily A1
    • Aldehyde dehydrogenase family 3, subfamily A, member 1
    • Aldehyde dehydrogenase isozyme 3
    • Aldehyde dehydrogenase type III
    • ALDH 3
    • ALDH 3A1
    • ALDH III
    • ALDH, stomach type
    • ALDH3
    • ALDH3 A1
    • ALDH3A 1
    • Aldh3a1
    • ALDHIII
    • dimeric NADP-preferring
    • MGC10406
    • Stomach aldehyde dehydrogenase
    • Tumor associated aldehyde dehydrogenase tumor ALDH
    see all
  • Function

    ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.
  • Tissue specificity

    High levels in stomach, esophagus and lung; low level in the liver and kidney.
  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab93463 (3µg).

References

ab93463 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your reply. The specific information activity for these products is as follows:


ab87415: Specific activity is > 0.14 units/ml

ab93463 and ab117154: Specific activity is > 0.5 units/ml

The protocol used to measure the specific activity is:

Activity Assay

1) Prepare a 3ml reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA.
2) Equilibrate to 25C and monitor the A340nm until value is constant using a spectrophotometer.
3) Add 5ug of recombinant ALDH2 into reaction mixture and mix immediately.
4) Record the increase in A340nm for 5 minutes



I hope this helps, please let me know if you need any additional information.

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