Recombinant Human ALKBH2 protein (ab105622)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Human ALKBH2 protein -
Purity
> 90 % SDS-PAGE.
ab105622 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMDRFLVKGAQGGLLRKQEEQEPTGEEPAVL GGDKESTRKRPRREAPGNGGHSAGPSWRHIRAEGLDCSYTVLFGKAEADE IFQELEKEVEYFTGALARVQVFGKWHSVPRKQATYGDAGLTYTFSGLTLS PKPWIPVLERIRDHVSGVTGQTFNFVLINRYKDGCDHIGEHRDDERELAP GSPIASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAHGSLLMMNHPT NTHWYHSLPVRKKVLAPRVNLTFRKILLTKK -
Predicted molecular weight
31 kDa including tags -
Amino acids
1 to 261 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab105622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Additional notes
Previously labelled as ABH2.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 30% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- 2OG Fe(II) oxy DC1
- 9530023G02
- ABH 2
see all -
Function
Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. -
Tissue specificity
Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder. -
Sequence similarities
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain. -
Cellular localization
Nucleus. Detected in replication foci during S-phase. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab105622 has not yet been referenced specifically in any publications.