Recombinant Human ALKBH2 protein (ab105622)

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SDS-PAGE - Recombinant Human ALKBH2 protein (ab105622)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human ALKBH2 protein

    • Purity

      > 90 % SDS-PAGE.
      ab105622 was purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      Q6NS38

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMDRFLVKGAQGGLLRKQEEQEPTGEEPAVL GGDKESTRKRPRREAPGNGGHSAGPSWRHIRAEGLDCSYTVLFGKAEADE IFQELEKEVEYFTGALARVQVFGKWHSVPRKQATYGDAGLTYTFSGLTLS PKPWIPVLERIRDHVSGVTGQTFNFVLINRYKDGCDHIGEHRDDERELAP GSPIASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAHGSLLMMNHPT NTHWYHSLPVRKKVLAPRVNLTFRKILLTKK

      • Predicted molecular weight

        31 kDa including tags

      • Amino acids

        1 to 261

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab105622 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid

    • Additional notes

      Previously labelled as ABH2. 

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.0154% DTT, 0.316% Tris HCl, 30% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • 2OG Fe(II) oxy DC1
      • 9530023G02
      • ABH 2
      • ABH2
      • alkB alkylation repair homolog 2
      • ALKB2_HUMAN
      • ALKBH2
      • ALKBH2 alkB
      • Alkylated DNA repair protein alkB homolog
      • Alkylated DNA repair protein alkB homolog 2
      • Alkylation repair homolog 2
      • Alpha ketoglutarate dependent dioxygenase alkB homolog 2
      • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2
      • AU016977
      • DNA oxidative demethylase ALKBH2
      • EC 1.14.11.
      • FLJ99103
      • hABH2
      • mABH2
      • MGC90512
      • Oxy DC1
      see all

    • Function

      Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.

    • Tissue specificity

      Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.

    • Sequence similarities

      Belongs to the alkB family.
      Contains 1 Fe2OG dioxygenase domain.

    • Cellular localization

      Nucleus. Detected in replication foci during S-phase.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human ALKBH2 protein (ab105622)
      SDS-PAGE - Recombinant Human ALKBH2 protein (ab105622)

      15% SDS-PAGE analysis of ab105622 (3µg)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab105622? Please let us know so that we can cite the reference in this datasheet.

    ab105622 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    This ABH2 protein lost activity after being stored at -20C for around 8 months.

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    Answer

    Thank you for your call today and letting us know about the trouble with ab105622.

    As we discussed, I'd be happy to give you a 20% discount on another vial of ab105622 or alternatively you can participate in our testing discount program. In this testing program, you would need to submit a review of ab105622 in a functional assay, and in exchange we will give you one free protein on a future order within the next 4 months.

    Please let me know which of these options you would like, and I will set it up promptly. Let me know if you have any further questions or if there is anything else that we can do for you.

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