Recombinant Human ALKBH2 protein (ab105622)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      Q6NS38
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMDRFLVKGAQGGLLRKQEEQEPTGEEPAVL GGDKESTRKRPRREAPGNGGHSAGPSWRHIRAEGLDCSYTVLFGKAEADE IFQELEKEVEYFTGALARVQVFGKWHSVPRKQATYGDAGLTYTFSGLTLS PKPWIPVLERIRDHVSGVTGQTFNFVLINRYKDGCDHIGEHRDDERELAP GSPIASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAHGSLLMMNHPT NTHWYHSLPVRKKVLAPRVNLTFRKILLTKK
    • Molecular weight
      31 kDa including tags
    • Amino acids
      1 to 261
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab105622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 90 % SDS-PAGE.
    ab105622 was purified using conventional chromatography techniques.
  • Form
    Liquid
  • Additional notes

    Previously labelled as ABH2. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 30% Glycerol

General Info

  • Alternative names
    • 2OG Fe(II) oxy DC1
    • 9530023G02
    • ABH 2
    • ABH2
    • alkB alkylation repair homolog 2
    • ALKB2_HUMAN
    • ALKBH2
    • ALKBH2 alkB
    • Alkylated DNA repair protein alkB homolog
    • Alkylated DNA repair protein alkB homolog 2
    • Alkylation repair homolog 2
    • Alpha ketoglutarate dependent dioxygenase alkB homolog 2
    • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2
    • AU016977
    • DNA oxidative demethylase ALKBH2
    • EC 1.14.11.
    • FLJ99103
    • hABH2
    • mABH2
    • MGC90512
    • Oxy DC1
    see all
  • Function
    Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
  • Tissue specificity
    Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.
  • Sequence similarities
    Belongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localization
    Nucleus. Detected in replication foci during S-phase.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human ALKBH2 protein (ab105622)
    SDS-PAGE - Recombinant Human ALKBH2 protein (ab105622)
    15% SDS-PAGE analysis of ab105622 (3µg)

References

ab105622 has not yet been referenced specifically in any publications.

Publishing research using ab105622? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

This ABH2 protein lost activity after being stored at -20C for around 8 months.

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Answer

Thank you for your call today and letting us know about the trouble with ab105622.

As we discussed, I'd be happy to give you a 20% discount on another vial of ab105622 or alternatively you can participate in our testing discount program. In this testing program, you would need to submit a review of ab105622 in a functional assay, and in exchange we will give you one free protein on a future order within the next 4 months.

Please let me know which of these options you would like, and I will set it up promptly. Let me know if you have any further questions or if there is anything else that we can do for you.

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