Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant Human alpha 1 Fetoprotein + PD1
Purity> 90 % SDS-PAGE.
Expressed in E.coli as inclusion bodies. ab190415 was refolded using “temperature shift inclusion body refolding” technology and chromatographically purified.
Expression systemEscherichia coli
Protein lengthProtein fragment
SequenceMTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVK DALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSE EGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIAR RHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSG GSNIEFPGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFV LNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRAR RNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAG QFQTLV
Predicted molecular weight40 kDa including tags
Amino acids21 to 170
Additional sequence informationExtracellular domain, with AFP tag (Human Alpha-Fetal Protein N-terminal domain: 1-198 aa) at N terminal end of PD1.
Our Abpromise guarantee covers the use of ab190415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab190415 may be used for:
- 1. In vitro pro-B and T cells differentiation regulation study with this protein as either coating matrix protein or soluble factor.
- 2. May be used for protein-protein interaction assay.
- 3. As potential diagnostic biomarker protein for monitoring NK, B and T cell activities in vivo.
- 4. As antigen for specific antibody production.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 0.32% Tris HCl
Sterile-filtered, contains NaCl, KCl, EDTA, arginine, DTT and Glycerol.
Cellular localizationalpha 1 Fetoprotein: Secreted. PD1: Membrane.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab190415 has not yet been referenced specifically in any publications.