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    recombinant-human-alpha-a-crystallincryaa-protein-ab48778.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Recombinant human alpha A Crystallin/CRYAA protein (ab48778)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

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SDS-PAGE - Recombinant human alpha A Crystallin/CRYAA protein (ab48778)
  • Western blot - Recombinant human alpha A Crystallin/CRYAA protein (ab48778)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE, WB

Description

  • Product name

    Recombinant human alpha A Crystallin/CRYAA protein
    See all alpha A Crystallin/CRYAA proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MDVTIQHPWF KRTLGPFYPS RLFDQFFGEG LFEYDLLPFL SSTISPYYRQ SLFRTVLDSG ISEVRSDRDK FVIFLDVKHF SPEDLTVKVQ DDFVEIHGKH NERQDDHGYI SREFHRRYRL PSNVDQSALS CSLSADGMLT FCGPKIQTGL DATHAERAIP VSREEKPTSA PSS
    • Amino acids

      1 to 173

Associated products

  • Related Products

    • Anti-Alpha A + alpha B Crystallin antibody (ab28163)

Specifications

Our Abpromise guarantee covers the use of ab48778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Additional notes

     This product was previously labelled as alpha A Crystallin

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.316% Tris HCl, 0.0292% EDTA, 0.29% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Acry 1
    • Alpha crystallin A chain
    • Alpha-crystallin A chain
    • CRYA 1
    • CRYA1
    • CRYAA
    • CRYAA_HUMAN
    • Crystallin alpha 1
    • Crystallin alpha A
    • Heat shock protein beta 4
    • Heat shock protein beta-4
    • HSPB 4
    • HspB4
    • short form
    • Zonular Central Nuclear Cataract
    see all
  • Function

    May contribute to the transparency and refractive index of the lens.
  • Involvement in disease

    Defects in CRYAA are a cause of cataract autosomal dominant (ADC) [MIM:604219]. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood.
  • Sequence similarities

    Belongs to the small heat shock protein (HSP20) family.
  • Post-translational
    modifications

    O-glycosylated; contains N-acetylglucosamine side chains.
    Deamidation of Asn-101 in lens occurs mostly during the first 30 years of age, followed by a small additional amount of deamidation (approximately 5%) during the next approximately 38 years, resulting in a maximum of approximately 50% deamidation during the lifetime of the individual.
    Phosphorylation on Ser-122 seems to be developmentally regulated. Absent in the first months of life, it appears during the first 12 years of human lifetime. The relative amount of phosphorylated form versus unphosphorylated form does not change over the lifetime of the individual.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Target information above from: UniProt accession P02489 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant human alpha A Crystallin/CRYAA protein (ab48778)
    SDS-PAGE - Recombinant human alpha A Crystallin/CRYAA protein (ab48778)

    14% SDS-PAGE gel loaded with ab48778,
    recombinant human alpha A Crystallin/CRYAA protein,
    predicted molecular weight 19.9 kDa

  • Western blot - Recombinant human alpha A Crystallin/CRYAA protein (ab48778)
    Western blot - Recombinant human alpha A Crystallin/CRYAA protein (ab48778)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab48778? Please let us know so that we can cite the reference in this datasheet.

ab48778 has been referenced in 2 publications.

  • Fan Q  et al. Identification of proteins that interact with alpha A-crystallin using a human proteome microarray. Mol Vis 20:117-24 (2014). PubMed: 24453475
  • Rao NA  et al. Small Heat Shock Protein aA-Crystallin Prevents Photoreceptor Degeneration in Experimental Autoimmune Uveitis. PLoS One 7:e33582 (2012). PubMed: 22479415

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