Description

  • Product name

    Recombinant Human Alpha B Crystallin protein
    See all Alpha B Crystallin proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR PPSFLRAPSW FDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVT AAPKK
    • Predicted molecular weight

      20 kDa
    • Amino acids

      1 to 175

Specifications

Our Abpromise guarantee covers the use of ab48779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.316% Tris HCl, 0.0292% EDTA, 0.29% Sodium chloride

General Info

  • Alternative names

    • AACRYA
    • Alpha B crystallin
    • Alpha crystallin B chain
    • Alpha(B)-crystallin
    • Alpha-crystallin B chain
    • CRYA2
    • Cryab
    • CRYAB_HUMAN
    • Crystallin alpha B
    • Crystallin alpha polypeptide 2
    • CTPP2
    • Heat shock 20 kD like protein
    • Heat shock protein beta 5
    • Heat shock protein beta-5
    • HspB5
    • Renal carcinoma antigen NY REN 27
    • Renal carcinoma antigen NY-REN-27
    • Rosenthal fiber component
    see all
  • Function

    May contribute to the transparency and refractive index of the lens.
  • Tissue specificity

    Lens as well as other tissues.
  • Involvement in disease

    Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
  • Sequence similarities

    Belongs to the small heat shock protein (HSP20) family.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt

Images

  • 14% SDS-PAGE gel loaded with ab48779,
    recombinant Human alpha B Crystallin,
    predicted molecular weight 20.1 kDa

References

This product has been referenced in:

  • Kötter S  et al. Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins. J Cell Biol 204:187-202 (2014). Read more (PubMed: 24421331) »
  • Rao NA  et al. Small Heat Shock Protein aA-Crystallin Prevents Photoreceptor Degeneration in Experimental Autoimmune Uveitis. PLoS One 7:e33582 (2012). Read more (PubMed: 22479415) »
See all 2 Publications for this product

Customer reviews and Q&As

Lab Manager

Excellent
Abreviews
Application
Western blot
"This protein is high quality, with good purity by SDS-PAGE and low aggregation by SEC. It’s nice that there are no tags that can complicate experiments. It also arrived really quickly. We will buy it again as a standard of pure, high quality alpha B crystallin." - JH

Abcam user community

Verified customer

Submitted Sep 20 2019

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