Recombinant Human AMF protein (ab87625)

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SDS-PAGE - Recombinant Human AMF protein (ab87625)
  • Western blot - Recombinant Human AMF protein (ab87625)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Suitable for: SDS-PAGE, WB

Description

  • Product name

    Recombinant Human AMF protein
    See all AMF proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    ab87625 is purified using conventional chromatography techniques. Endotoxin: < 1.0 EU per 1 µg of protein (determined by LAL method).

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHH SSGLVPRGSH MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ

Specifications

Our Abpromise guarantee covers the use of ab87625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Form

    Liquid

  • Additional notes

     This product was previously labelled as Glucose 6 phosphate isomerase

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)

General Info

  • Alternative names

    • AMF
    • Aurocrine motility factor
    • Autocrine motility factor
    • DKFZp686C13233
    • EC 5.3.1.9
    • G6PI_HUMAN
    • Glucose phosphate isomerase
    • Glucose-6-phosphate isomerase
    • GNPI
    • GPI
    • Gpi1
    • Hexose monophosphate isomerase
    • Hexosephosphate isomerase
    • Neuroleukin
    • NLK
    • Oxoisomerase
    • PGI
    • PHI
    • Phosphoglucose isomerase
    • Phosphohexomutase
    • Phosphohexose isomerase
    • Phosphosaccharomutase
    • SA 36
    • SA-36
    • SA36
    • Sperm antigen 36
    see all

  • Function

    Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.

  • Pathway

    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.

  • Involvement in disease

    Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

  • Sequence similarities

    Belongs to the GPI family.

  • Post-translational
    modifications

    Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
    ISGylated.

  • Cellular localization

    Cytoplasm. Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human AMF protein (ab87625)
    SDS-PAGE - Recombinant Human AMF protein (ab87625)
    15% SDS-PAGE showing ab87625 at approximately 65kDa (3µg).
  • Western blot - Recombinant Human AMF protein (ab87625)
    Western blot - Recombinant Human AMF protein (ab87625)
    All lanes : Anti-AMF antibody (ab86950) at 1 µg/ml

    Lane 1 : Recombinant Human AMF protein (ab87625) at 0.1 µg
    Lane 2 : Recombinant Human AMF protein (ab87625) at 0.01 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 30 seconds

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (3)

Publishing research using ab87625? Please let us know so that we can cite the reference in this datasheet.

ab87625 has been referenced in 3 publications.

  • Finelli MJ  et al. Oxidation Resistance 1 Modulates Glycolytic Pathways in the Cerebellum via an Interaction with Glucose-6-Phosphate Isomerase. Mol Neurobiol N/A:N/A (2018). PubMed: 29905912
  • Tian K  et al. Microfluidics-based optimization of neuroleukin-mediated regulation of articular chondrocyte proliferation. Mol Med Rep 13:67-74 (2016). PubMed: 26573126
  • Zong M  et al. Glucose-6-phosphate isomerase promotes the proliferation and inhibits the apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis. Arthritis Res Ther 17:100 (2015). PubMed: 25889507

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