Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, WB
Product nameRecombinant Human AMF protein
See all AMF proteins and peptides
Purity> 95 % SDS-PAGE.
ab87625 is purified using conventional chromatography techniques. Endotoxin: < 1.0 EU per 1 µg of protein (determined by LAL method).
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMGSSHHHHHH SSGLVPRGSH MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ
Our Abpromise guarantee covers the use of ab87625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as Glucose 6 phosphate isomerase
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)
- Aurocrine motility factor
- Autocrine motility factor
FunctionBesides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
Involvement in diseaseDefects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Sequence similaritiesBelongs to the GPI family.
modificationsPhosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
Cellular localizationCytoplasm. Secreted.
- Information by UniProt
15% SDS-PAGE showing ab87625 at approximately 65kDa (3µg).
All lanes : Anti-AMF antibody (ab86950) at 1 µg/ml
Lane 1 :
Recombinant Human AMF protein (ab87625) at 0.1 µg
Lane 2 :
Recombinant Human AMF protein (ab87625) at 0.01 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab87625 has been referenced in 3 publications.
- Finelli MJ et al. Oxidation Resistance 1 Modulates Glycolytic Pathways in the Cerebellum via an Interaction with Glucose-6-Phosphate Isomerase. Mol Neurobiol N/A:N/A (2018). PubMed: 29905912
- Tian K et al. Microfluidics-based optimization of neuroleukin-mediated regulation of articular chondrocyte proliferation. Mol Med Rep 13:67-74 (2016). PubMed: 26573126
- Zong M et al. Glucose-6-phosphate isomerase promotes the proliferation and inhibits the apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis. Arthritis Res Ther 17:100 (2015). PubMed: 25889507