Overview

  • Product name

    Recombinant Human AMG protein
  • Protein length

    Full length protein

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMPLPPHP GHPGYINFSY EVLTPLKWYQ SIRPPYPSYG YEPMGGWLHH QIIPVLSQQH PPTHTLQPHH HIPVVPAQQP VIPQQPMMPV PGQHSMTPIQ HHQPNLPPPA QQPYQPQPVQ PQPHQPMQPQ PPVHPMQPLP PQPPLPPMFP MQPLPPMLPD LTLEAWPSTD KTKREEVD
    • Molecular weight

      22 kDa including tags
    • Amino acids

      17 to 191
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab139212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF
  • Purity

    > 90 % SDS-PAGE.
    ab139212 was purified using conventional chromatography techniques.
  • Form

    Liquid
  • Additional notes

     This product was previously labelled as AMELX

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.50
    Constituents: 0.02% DTT, 0.32% Tris HCl, 30% Glycerol, 1.17% Sodium chloride

General Info

  • Alternative names

    • AI1E
    • AIH1
    • ALGN
    • Amel
    • Amelogenesis imperfecta 1
    • Amelogenin
    • Amelogenin (amelogenesis imperfecta 1, X linked)
    • Amelogenin (X chromosome)
    • Amelogenin (X chromosome, amelogenesis imperfecta 1)
    • Amelogenin X isoform
    • Amelogenin, X linked
    • AMELX
    • AMELX_HUMAN
    • Amg
    • AMGL
    • AMGX
    • OTTHUMP00000022906
    • OTTHUMP00000022907
    • X isoform
    see all
  • Function

    Plays a role in biomineralization. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel.
  • Involvement in disease

    Defects in AMELX are the cause of amelogenesis imperfecta hypoplastic type 1 (AIH1) [MIM:301200]. AIH1 is a X-linked defect of dental enamel formation. Teeth have only a thin layer of enamel with normal hardness. The thinness of the enamel makes the teeth appear small.
  • Sequence similarities

    Belongs to the amelogenin family.
  • Developmental stage

    Transiently but abundantly expressed by ameloblasts during tooth development. Amelogenin is the predominant protein in developing dental enamel.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab139212 (3µg)

References

This product has been referenced in:

  • Jiang T  et al. Distribution of Cathepsin K in Late Stage of Tooth Germ Development and Its Function in Degrading Enamel Matrix Proteins in Mouse. PLoS One 12:e0169857 (2017). Read more (PubMed: 28095448) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you very much for contacting us with your enquiry.

The AMELX protein ab139212 can be diluted in any buffer. However, all of our products are intended for research use only and are not recommended for in vitro studies. This protein contains DTT, glycerol, and Tris HCL in its buffer, and it is not a sterile product, so we do not suggest it for use in a live animal study.

Ab13420 and ab90395 are mouse monoclonal antibodies, so they can be detected with a detection kit like ab94743-

https://www.abcam.com/EXPOSE-Mouse-Specific-AP-red-Detection-IHC-Kit-ab94743.html

I hope that this information will be useful, but please let me know if you have further questions or need anything else, and I'll be happy to help.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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