The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml.
< 0.100 Eu/µg
% SDS-PAGE. Purity is > 95% by SDS-PAGE gel and HPLC analyses.
Sterile filtered through a 0.2 micron filter.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.00 Constituent: 0.082% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Colorectum cell derived growth factor
Colorectum cell-derived growth factor
Schwannoma derived growth factor
Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells.
Belongs to the amphiregulin family. Contains 1 EGF-like domain.