Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, WB
Product nameRecombinant human Amphiregulin protein
See all Amphiregulin proteins and peptides
Biological activityDetermined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml.
Purity> 95 % SDS-PAGE.
Purity is > 95% by SDS-PAGE gel and HPLC analyses. Sterile filtered through a 0.2 micron filter.
Endotoxin level< 0.100 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight11 kDa
Amino acids101 to 198
Our Abpromise guarantee covers the use of ab104355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
Constituent: 0.082% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
- A REG
- Amphiregulin B
FunctionBifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells.
Sequence similaritiesBelongs to the amphiregulin family.
Contains 1 EGF-like domain.
- Information by UniProt
Anti-Amphiregulin antibody [MM0089-8K16] (ab89119) at 1/500 dilution +
Recombinant human Amphiregulin protein (ab104355) at 0.1 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 8 minutes
ab89119 recognizes the recombinant full length soluble Human Amphiregulin protein (ab104355) which has an expected molecular weight of ~12 kDa.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab104355 has been referenced in 1 publication.
- Hobor S et al. TGFa and amphiregulin paracrine network promotes resistance to EGFR blockade in colorectal cancer cells. Clin Cancer Res 20:6429-38 (2014). PubMed: 24916700