Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human Amphiregulin protein (Active)
See all Amphiregulin proteins and peptides
3T3 Cell Proliferation.
ED50 ≤ 20 ng/ml (≥ 5.0 x 104 units/mg).
Purity>= 95 % SDS-PAGE.
Purity determined by reducing and non-reducing SDS-PAGE.
Endotoxin level<=1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight10 kDa
Amino acids101 to 187
Additional sequence informationThis product is the mature full length protein from aa 101 to 187. The signal peptide and propeptide are not included.
Our Abpromise guarantee covers the use of ab229600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: 0.16% Sodium phosphate
Lyophilized from a sterile (0.2 micron) filtered aqueous solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile water at 0.1 mg/ml. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
- A REG
- Amphiregulin B
FunctionBifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells.
Sequence similaritiesBelongs to the amphiregulin family.
Contains 1 EGF-like domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229600 has not yet been referenced specifically in any publications.