Product nameRecombinant human AMPK alpha 1 + AMPK beta 2 + AMPK gamma 3 protein (Active)
Protein lengthFull length protein
SourceBaculovirus infected Sf9 cells
Amino Acid Sequence
TagsHis tag C-Terminus , proprietary tag N-Terminus
Additional sequence informationRecombinant full length AMPK alpha 1 (a.a's 1-559 of UniProt ID: Q13131) + AMPK beta 2 (a.a's 1-272 of UniProt ID: O43741) + AMPK gamma 3 (a.a.'s 1- 489 of UniProt ID:Q9UGI9). MWt 92, 62, and 108 KDa.
Our Abpromise guarantee covers the use of ab184885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The specific activity of ab184885 was determined to be 400 nmol/min/mg
Purity> 80 % Densitometry.
ab204881 (Acetyl Coenzyme A Carboxylase peptide) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 5 AMP activated protein kinase alpha 1catalytic subunit
- 5 AMP activated protein kinase catalytic alpha 1 chain
- 5 AMP activated protein kinase subunit gamma 3
RelevanceAMPK alpha 1: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. AMPK beta 2: AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. Also regulates cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase and hormone-sensitive lipase. This is a regulatory subunit, may be a positive regulator of AMPK activity. It may also serve as an adapter molecule for the catalytic alpha-subunit. AMPK gamma 3: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
Cellular localizationCytoplasmic and Nuclear
ab184885 has not yet been referenced specifically in any publications.