Recombinant human AMPK alpha 2 + PRKAG3 + AMPK beta 2/PRKAB2 protein (Active) (ab268342)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, GST tag N-Terminus, His tag C-Terminus, GST tag N-Terminus, His tag C-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human AMPK alpha 2 + PRKAG3 + AMPK beta 2/PRKAB2 protein (Active) -
Biological activity
The specific activity of ab268342 was 58 nmol/min/min in a peptide kinase assay using SAMStide (HMRSAMSGLHLVKRR) as substrate.
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Purity
> 80 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQ KIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGE LFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAH MNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSC GVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQ VDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKF ECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGS FMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKA KWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNY VKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRS SFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTL AR -
Molecular weight information
SDS-PAGE molecular weight: ~92 kDa -
Amino acids
1 to 552 -
Tags
His tag C-Terminus , GST tag N-Terminus -
Additional sequence information
GeneBank: NM_006252
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Amino Acid Sequence 2
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Species
Human -
Sequence
MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPSVFSLPD SKLPGDKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWS TKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTIN NLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEE RFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSV MVLSATHRYKKKYVTTLLYKPI -
Molecular weight information
SDS-PAGE molecular weight: ~62 kDa -
Amino acids
1 to 272 -
Tags
His tag C-Terminus , GST tag N-Terminus -
Additional sequence information
GeneBank: NM_005399
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Amino Acid Sequence 3
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Species
Human -
Sequence
MEPGLEHALRRTPSWSSLGGSEHQEMSFLEQENSSSWPSPAVTSSSERIR GKRRAKALRWTRQKSVEEGEPPGQGEGPRSRPAAESTGLEATFPKTTPLA QADPAGVGTPPTGWDCLPSDCTASAAGSSTDDVELATEFPATEAWECELE GLLEERPALCLSPQAPFPKLGWDDELRKPGAQIYMRFMQEHTCYDAMATS SKLVIFDTMLEIKKAFFALVANGVRAAPLWDSKKQSFVGMLTITDFILVL HRYYRSPLVQIYEIEQHKIETWREIYLQGCFKPLVSISPNDSLFEAVYTL IKNRIHRLPVLDPVSGNVLHILTHKRLLKFLHIFGSLLPRPSFLYRTIQD LGIGTFRDLAVVLETAPILTALDIFVDRRVSALPVVNECGQVVGLYSRFD VIHLAAQQTYNHLDMSVGEALRQRTLCLEGVLSCQPHESLGEVIDRIARE QVHRLVLVDETQHLLGVVSLSDILQALVLSPAGIDALGA -
Molecular weight information
SDS-PAGE molecular weight: ~108 kDa -
Amino acids
1 to 489 -
Tags
His tag C-Terminus , GST tag N-Terminus -
Additional sequence information
GeneBank: NM_017431
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab268342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Cellular localization
AMPK alpha 2: Cytoplasm. Nucleus. In response to stress, recruited by p53/TP53 to specific promoters.
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab268342 has not yet been referenced specifically in any publications.