Recombinant human AMPK alpha1 + AMPK beta1 + AMPK gamma1 protein (Tagged) (ab271362)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, His tag C-Terminus, His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human AMPK alpha1 + AMPK beta1 + AMPK gamma1 protein (Tagged)
See all AMPK alpha1 + AMPK beta1 + AMPK gamma1 proteins and peptides -
Biological activity
Specific Activity: ≥300 pmole/min/µgAssay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using AMARA peptide as a substrate (0.1 mg/ml), 20 μM ATP and 100 μM AMP . Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase- Glo reagent. -
Purity
>= 90 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTG HKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDI FMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRD LKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRL YAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNP SVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMID DEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKD FYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQ GVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNP VTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNY RSCQRSDSDAEAQGKSSEVSLTSSVTSLDSSPVDLTPRPGSHTIEFFEMC ANLIKILAQ -
Molecular weight information
65 kDa by SDS-PAGE ~146 kDa (complex) -
Amino acids
1 to 559 -
Tags
His tag C-Terminus -
Additional sequence information
AMPK alpha1 protein
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Amino Acid Sequence 2
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Species
Human -
Sequence
GNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEE IKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWS KLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNN IIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFR APPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVL SATHRYKKKYVTTLLYKPI -
Molecular weight information
31 kDa by SDS-PAGE ~146 kDa (complex) -
Amino acids
2 to 270 -
Tags
His tag C-Terminus
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Amino Acid Sequence 3
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Species
Human -
Sequence
METVISSDSSPAVENEHPQETPESNNSVYTSFMKSHRCYDLIPTSSKLVV FDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYK SALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKI HRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGT YANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLA AEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRL VVVDENDVVKGIVSLSDILQALVLTGGEKKP -
Molecular weight information
38 kDa by SDS-PAGE ~146 kDa (complex) -
Amino acids
1 to 331 -
Tags
His tag C-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab271362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.82% Sodium phosphate, 1.74% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 5' AMP activated protein kinase catalytic subunit alpha 1
- 5' AMP activated protein kinase non catalytic subunit beta 1
- 5' AMP activated protein kinase non catalytic subunit gamma 1
see all -
Relevance
AMPK (5'-prime-AMP-activated protein) is a heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic regulatory subunits. It belongs to the ser/thr protein kinase family. AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is also responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It regulates cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase and hormone-sensitive lipase.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab271362 has not yet been referenced specifically in any publications.