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I have an anti-His tag antibody that will only recognize HHHHHHGS, and not HHHHHH alone. Does this protein sequence have HHHHHHGS? Plus is the His tag N-terminal or C-terminal on this protein?
Asked on Sep 23 2011
Thank you for your inquiry. The Androgen Receptor ab82124 has a Flag tag on the N-terminus (I'll be sure to update the datasheet with this information) and a 8x His-tag on C terminus. There is no 6xH+GS sequence with this protein so I'm not sure if the antibody you have will work. I hope this information helps. Please contact us with any other questions.
Answered on Sep 23 2011