Recombinant Human Angiogenin protein (denatured) (ab177677)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Angiogenin protein (denatured)
See all Angiogenin proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHH SSGLVPRGSH MQDNSRYTHF LTQHYDAKPQ GRDDRYCESI MRRRGLTSPC KDINTFIHGN KRSIKAICEN KNGNPHRENL RISKSSFQVT TCKLHGGSPW PPCQYRATAG FRNVVVACEN GLPVHLDQSI FRRP -
Predicted molecular weight
16 kDa including tags -
Amino acids
25 to 147 -
Tags
His tag N-Terminus -
Additional sequence information
Mature protein. NP_001136.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab177677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 2.4% Urea, 10% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- ALS9
- AMYOTROPHIC LATERAL SCLEROSIS
- ANG
see all -
Function
May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. -
Tissue specificity
Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. -
Involvement in disease
Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9) [MIM:611895]. ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. -
Sequence similarities
Belongs to the pancreatic ribonuclease family. -
Developmental stage
Low level expression in the developing fetus, increased in the neonate, and maximal in the adult. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human Angiogenin protein (denatured) (ab177677)
15% SDS-PAGE analysis of ab177677 (3μg).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab177677 has not yet been referenced specifically in any publications.
