Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MRGSHHHHHH GMASHMSRID QDNSSFDSLS PEPKSRFAML DDVKILANGL LQLGHGLKDF VHKTKGQIND IFQKLNIFDQ SFYDLSLQTS EIKEEEKELR RTTYKLQVKN EEVKNMSLEL NSKLESLLEE KILLQQKVKY LEEQLTNLIQ NQPETPEHPE VTSLKTFVEK QDNSIKDLLQ TVEDQYKQLN QQHSQIKEIE NQLRRTSIQE PTEISLSSKP RAP
    • Molecular weight

      26 kDa including tags
    • Amino acids

      17 to 223
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab203531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Effects of ANGPTL3 on serum levels of triglycerides, on blood glucose and on serum cholesterol levels in C57BL/6J mice.

  • Applications

    SDS-PAGE

    Functional Studies

    Western blot

  • Endotoxin level

    < 1.000 Eu/µg
  • Purity

    > 95 % SDS-PAGE.
    Assessed by densitometry. Purifed using Ni-NTA chromatography.
  • Form

    Lyophilised
  • Additional notes

    For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    pH: 4.00
    Constituent: 0.68% Sodium acetate trihydrate

    Lyophilized from a 0.4 µM filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

General Info

  • Alternative names

    • ANG 5
    • ANG-5
    • ANG5
    • Angiopoietin 5
    • Angiopoietin like 3
    • Angiopoietin related protein 3
    • Angiopoietin-5
    • Angiopoietin-like protein 3
    • Angiopoietin-related protein 3
    • ANGL3_HUMAN
    • ANGPT5
    • ANGPTL3
    • ANL3
    • FHBL2
    • OTTHUMP00000010719
    • UNQ153/PRO179
    see all
  • Tissue specificity

    Expressed principally in liver. Weakly expressed in kidney.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • 12% SDS-PAGE analysis of ab203531.

    Lane 1: Reduced and heated sample, 5 μg/lane
    Lane 2: Non-reduced and non-heated sample, 5 μg/lane

References

ab203531 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab203531.
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