Recombinant human ANGPTL3 protein (Tagged) (ab271363)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
-
Product name
Recombinant human ANGPTL3 protein (Tagged)
See all ANGPTL3 proteins and peptides -
Biological activity
ANGPTL3 activity was confirmed by treating THP-1 cells with 500 ng/ml of ANGPTL3 for 15 min, 30 min, 2 hr, or 4 hr. Phosphorylated-Erk was measured by Western blot and compared to total Erk levels.
-
Purity
>= 90 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MFTIKLLLFI VPLVISSRID QDNSSFDSLS PEPKSRFAML DDVKILANGL LQLGHGLKDF VHKTKGQIND IFQKLNIFDQ SFYDLSLQTS EIKEEEKELR RTTYKLQVKN EEVKNMSLEL NSKLESLLEE KILLQQKVKY LEEQLTNLIQ NQPETPEHPE VTSLKTFVEK QDNSIKDLLQ TVEDQYKQLN QQHSQIKEIE NQLRRTSIQE PTEISLSSKP RAPRTTPFLQ LNEIRNVKHD GIPAECTTIY NRGEHTSGMY AIRPSNSQVF HVYCDVISGS PWTLIQHRID GSQNFNETWE NYKYGFGRLD GEFWLGLEKI YSIVKQSNYV LRIELEDWKD NKHYIEYSFY LGNHETNYTL HLVAITGNVP NAIPENKDLV FSTWDHKAKG HFNCPEGYSG GWWWHDECGE NNLNGKYNKP RAKSKPERRR GLSWKSQNGR LYSIKSTKML IHPTDSESFE -
Actual molecular weight
53 kDa including tags -
Amino acids
1 to 460 -
Modifications
biotinylated -
Tags
DDDDK tag C-Terminus -
Additional sequence information
Genbank: NM_014495.3
-
Specifications
Our Abpromise guarantee covers the use of ab271363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
SDS-PAGE
-
Form
Lyophilized -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 0.58% Sodium chloride, 0.02% Potassium chloride, 0.13% Sodium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionWe recommend dissolving in water. Store in aliquots at -80C. Keep final concentration for storage at least 10 ug/ml or higher.
General Info
-
Alternative names
- ANG 5
- ANG-5
- ANG5
see all -
Tissue specificity
Expressed principally in liver. Weakly expressed in kidney. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Cellular localization
Secreted. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab271363 has not yet been referenced specifically in any publications.