Recombinant human ANKK1 protein (ab204164)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human ANKK1 protein -
Biological activity
The Specific activity of ab204164 was determined to be 80 nmol/min/mg.
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Purity
> 75 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAADPTELRLGSLPVFTRDDFEGDWRLVASGGFSQVFQARHRRWRTEYAI KCAPCLPPDAASSDVNYLIEEAAKMKKIKFQHIVSIYGVCKQPLGIVMEF MANGSLEKVLSTHSLCWKLRFRIIHETSLAMNFLHSIKPPLLHLDLKPGN ILLDSNMHVKISDFGLSKWMEQSTRMQYIERSALRGMLSYIPPEMFLESN KAPGPKYDVYSFAIVIWELLTQKKPYSGFNMMMIIIRVAA GMRPSLQP VSDQWPSEAQQMVDLMKRCWDQDPKKRPCFLDITIETDILLSLLQSRVAV PESKALARKVSCKLSLRQPGEVNEDISQELMDSDSGNYLKRALQLSDRKN LVPRDEELCIYENKVTPLHFLVAQGSVEQVRLLLAHEVDVDCQTASGYTP LLIAAQDQQPDLCALLLAHGADANRVDEDGWAPLHFAAQNGDDGTARLLL DHGACVDAQEREGWTPLHLAAQNNFENVARLLVSRQADPNLHEAEGKTPL HVAAYFGHVSLVKLLTSQGAELDAQQRNLRTPLHLAVERGKVRAIQHLLK SGAVPDALDQSGYGPLHTAAARGKYLICKMLLRYGASLELPTHQGWTPLH LAAYKGHLEIIHLLAESHANMGALGAVNWTPLHLAARHGEEAVVSALLQC GADPNAAEQSGWTPLHLAVQRSTFLSVINLLEHHANVHARNKVGWTPAHL AALKGNTAILKVLVEAGAQLDVQDGVSCTPLQLALRSRKQGIMSFLEGKE PSVATLGGSKPGAEMEI -
Predicted molecular weight
115 kDa including tags -
Amino acids
1 to 765 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_178510.
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab204164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.002% PMSF, 0.004% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Ankyrin repeat and kinase domain containing 1
- Ankyrin repeat and protein kinase domain containing protein 1
- EC 2.7.11.1
see all -
Relevance
ANKK1 belongs to the TKL Ser/Thr protein kinase superfamily, contains twelve ANK repeats and one protein kinase domain. The protein is weakly expressed in testis, placenta, and spinal cord.
Images
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The specific activity of ANKK1 (ab204164) was determined to be 1 nmol/min/mg as per activity assay protocol and was equivalent to 7 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab204164
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SDS PAGE analysis of ab204164
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Analysis of the purity of ab204164 by densitometry.
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Kinase assay demonstrating specific activity of ab204164.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab204164 has not yet been referenced specifically in any publications.