Description

Specifications

Our Abpromise guarantee covers the use of ab93005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

    Western blot

    ELISA

  • Form

    Liquid
  • Additional notes

     This product was previously labelled as Annexin A2

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Preservative: 0.68% Imidazole
    Constituents: 0.0078% Beta mercaptoethanol, 0.316% Tris HCl, 2.9% Sodium chloride

General Info

  • Alternative names

    • Annexin A2
    • Annexin II
    • Annexin II, heavy chain
    • Annexin-2
    • ANX 2
    • ANX2
    • ANX2L4
    • ANXA2
    • ANXA2_HUMAN
    • arylsulfatase B
    • CAL1H
    • Calpactin I heavy chain
    • Calpactin I heavy polypeptide
    • calpactin I heavy polypeptide (p36)
    • Calpactin-1 heavy chain
    • chromobindin 8
    • Chromobindin-8
    • Epididymis secretory protein Li 270
    • HEL S 270
    • LIP2
    • Lipocortin II
    • LPC2
    • LPC2D
    • p36
    • P36 protein
    • PAP-IV
    • Placental anticoagulant protein IV
    • Protein I
    see all
  • Function

    Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt

Images

  • ab93005 analysed by 10% SDS-PAGE stained with Coomassie Blue (CB) and immunoblotted with anti-6x His serum (WB).

References

ab93005 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
Application
ELISA
We coated 0.2 ug of this protein in carbonate-bicarbonate buffer (pH9.5) into each well on 96-well plate (high binding, Corning) for ELISA assay, the coated annexin a2 (ANXA2) worked very well for ELISA assay.

Abcam user community

Verified customer

Submitted Nov 05 2015

Abreviews
Application
Functional Studies
We co-incubated this protein and its interacting partner for 2 hours at RT followed by native band shift electrophoresis and immunological blot assays, the result showed that this ANXA2 protein is extremely stable and functional for the assay.

Abcam user community

Verified customer

Submitted Nov 02 2015

Answer

Thank you for contacting us. Unfortunately, the Annexin II proteins ab93005 and ab81777 were not tested for their biological activity. Also, as the purification process may influence the conformation of the protein, we cannot guarantee that the Annexin II proteins would exhibit biological activity. I am sorry that I cannot be of more assistance on this occasion. Please do not hesitate to contact us if you need anything further.

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