Key features and details
- Expression system: Escherichia coli
- Purity: >= 89% HPLC
- Active: Yes
- Suitable for: Flow Cyt, SDS-PAGE, HPLC
Product nameRecombinant human Annexin V/ANXA5 protein (PE)
See all Annexin V/ANXA5 proteins and peptides
Binds to phosphatidylserine (PS). Exhibits anti-phospholipase activity.
Purity>= 89 % HPLC.
ab157016 was determined to be >= 89% pure by SDS-PAGE and HPLC.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQR QEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHAL KGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQ RMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGT RSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAY LAETLYYAMKGAGTDDHTLIRVMVSRSEIDLFNIRKEFRKNFATSLYSMI KGDTSGDYKKALLLLCGEDD
Predicted molecular weight36 kDa
Amino acids1 to 320
ConjugationPE. Ex: 488nm, Em: 575nm
Our Abpromise guarantee covers the use of ab157016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesab157016 can be used for detection of apoptotic cells by flow cytometry. It is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable secondary antibody.
This product was previously labelled as Annexin V
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.
Preservative: 0.02% Sodium azide
Constituents: 0.61% Tris, 0.58% Sodium chloride, 1% BSA
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Anchorin CII
- Annexin 5
- Annexin A5
FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Involvement in diseasePregnancy loss, recurrent, 3
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid.
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab157016 has not yet been referenced specifically in any publications.