Recombinant human Annexin V/ANXA5 protein (PE) (ab157016)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 89% HPLC
- Active: Yes
- Suitable for: Flow Cyt, SDS-PAGE, HPLC
Description
-
Product name
Recombinant human Annexin V/ANXA5 protein (PE)
See all Annexin V/ANXA5 proteins and peptides -
Biological activity
Binds to phosphatidylserine (PS). Exhibits anti-phospholipase activity.
-
Purity
>= 89 % HPLC.
ab157016 was determined to be >= 89% pure by SDS-PAGE and HPLC. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQR QEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHAL KGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQ RMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGT RSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAY LAETLYYAMKGAGTDDHTLIRVMVSRSEIDLFNIRKEFRKNFATSLYSMI KGDTSGDYKKALLLLCGEDD -
Predicted molecular weight
36 kDa -
Amino acids
1 to 320
-
-
Conjugation
PE. Ex: 488nm, Em: 575nm
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab157016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Flow Cytometry
SDS-PAGE
HPLC
-
Form
Liquid -
Additional notes
ab157016 can be used for detection of apoptotic cells by flow cytometry. It is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable secondary antibody.This product was previously labelled as Annexin V
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.
Preservative: 0.02% Sodium azide
Constituents: 0.61% Tris, 0.58% Sodium chloride, 1% BSAThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all -
Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab157016 has not yet been referenced specifically in any publications.