Recombinant human Antithrombin III/ATIII protein (Active) (ab234969)

Overview

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected insect cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      ADPHGSPVDICTAKPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRR VWELSKANSRFATTFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACND TLQQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSKLVSANRLF GDKSLTFNETYQDISELVYGAKLQPLDFKENAEQSRAAINKWVSNKTEGR ITDVIPSEAINELTVLVLVNTIYFKGLWKSKFSPENTRKELFYKADGESC SASMMYQEGKFRYRRVAEGTQVLELPFKGDDITMVLILPKPEKSLAKVEK ELTPEVLQEWLDELEEMMLVVHMPRFRIEDGFSLKEQLQDMGLVDLFSPE KSKLPGIVAEGRDDLYVSDAFHKAFLEVNEEGSEAAASTAVVIAGRSLNP NRVTFKANRPFLVFIREVPLNTIIFMGRVANPCVKHHHHHH
    • Molecular weight

      52 kDa
    • Amino acids

      33 to 464
    • Tags

      His tag C-Terminus
    • Additional sequence information

      This product is the mature full length protein from aa 33 to 464. The signal peptide is not included (NP_000479).

Specifications

Our Abpromise guarantee covers the use of ab234969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Measured by its ability to inhibit Thrombin cleavage of substrate Boc-VPR-AMC. The IC50 for this effect is less or equal to 1 nM.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level

    < 1.000 Eu/µg
  • Purity

    > 90 % SDS-PAGE.
    Purified by using conventional chromatography techniques.
  • Form

    Liquid
  • Additional notes

    Previously labelled as Antithrombin III.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: PBS, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • ANT3_HUMAN
    • Antithrombin
    • Antithrombin III
    • Antithrombin-III
    • AntithrombinIII
    • AT 3
    • AT III
    • AT3
    • AT3D
    • ATIII
    • Heparin cofactor I
    • MGC22579
    • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1
    • Serine cysteine proteinase inhibitor clade C member 1
    • Serine proteinase inhibitor clade C member 1
    • Serpin C1
    • Serpin family C member 1
    • Serpin peptidase inhibitor clade C (antithrombin) member 1
    • SERPINC1
    • THPH7
    see all
  • Function

    Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Tissue specificity

    Found in plasma.
  • Involvement in disease

    Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3 µg ab234969.

    MW: 50-70 kDa (SDS-PAGE under reducing conditions).

References

ab234969 has not yet been referenced specifically in any publications.

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