Recombinant Human APOA4/Apo-AIV protein (ab132653)

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Overview

Description

  • Nature

    Recombinant

  • Source

    Wheat germ
  • Amino Acid Sequence
    • Accession
      P06727

    • Species

      Human

    • Sequence

      MFLKAVVLTLALVAVAGARAEVSADQVATVMWDYFSQLSNNAKEAVEHLQ KSELTQQLNALFQDKLGEVNTYAGDLQKKLVPFATELHERLAKDSEKLKE EIGKELEELRARLLPHANEVSQKIGDNLRELQQRLEPYADQLRTQVNTQA EQLRRQLTPYAQRMERVLRENADSLQASLRPHADELKAKIDQNVEELKGR LTPYADEFKVKIDQTVEELRRSLAPYAQDTQEKLNHQLEGLTFQMKKNAE ELKARISASAEELRQRLAPLAEDVRGNLRGNTEGLQKSLAELGGHLDQQV EEFRRRVEPYGENFNKALVQQMEQLRQKLGPHAGDVEGHLSFLEKDLRDK VNSFFSTFKEKESQDKTLSLPELEQQQEQQQEQQQEQVQMLAPLES

    • Molecular weight

      72 kDa including tags

    • Amino acids

      1 to 396

Specifications

Our Abpromise guarantee covers the use of ab132653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Western blot

  • Form

    Liquid

  • Additional notes

    Protein concentration is above or equal to 0.05 mg/ml. Best used within three months from the date of receipt.

     This product was previously labelled as APOA4

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • Apo AIV
    • APOA 4
    • ApoA IV
    • APOA4
    • Apolipoprotein A IV
    • Apolipoprotein A4
    • MGC142154
    • MGC142156
    see all

  • Relevance

    APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties

  • Cellular localization

    Secreted

Images

  • SDS-PAGE - Recombinant Human APOA4/Apo-AIV protein (ab132653)
    SDS-PAGE - Recombinant Human APOA4/Apo-AIV protein (ab132653)
    12.5% SDS-PAGE analysis of ab132653 stained with Coomassie Blue.

References

ab132653 has not yet been referenced specifically in any publications.

Publishing research using ab132653? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Our lab will be needing to order purified apoa4 in the near future. Could you tell me how long it would take to receive this item (ab132653) once it is ordered? We Our intent is to use it in in vitro tissue culture – is the protein you provide active and suitable for this purpose?

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Answer



We don't currently have this protein in stock, but once it's ordered, it will take between 2 and 3 weeks to arrive.

At this time we have not tested the functionality of the protein and we do not know if it is an active protein. It has been used as a control in Western blot and ELISA, but not in tissue culture.

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