Recombinant Human APOA4/Apo-AIV protein (ab132653)

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SDS-PAGE - Recombinant Human APOA4/Apo-AIV protein (ab132653)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human APOA4/Apo-AIV protein
      See all APOA4/Apo-AIV proteins and peptides

    • Expression system

      Wheat germ

    • Accession

      P06727

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MFLKAVVLTLALVAVAGARAEVSADQVATVMWDYFSQLSNNAKEAVEHLQ KSELTQQLNALFQDKLGEVNTYAGDLQKKLVPFATELHERLAKDSEKLKE EIGKELEELRARLLPHANEVSQKIGDNLRELQQRLEPYADQLRTQVNTQA EQLRRQLTPYAQRMERVLRENADSLQASLRPHADELKAKIDQNVEELKGR LTPYADEFKVKIDQTVEELRRSLAPYAQDTQEKLNHQLEGLTFQMKKNAE ELKARISASAEELRQRLAPLAEDVRGNLRGNTEGLQKSLAELGGHLDQQV EEFRRRVEPYGENFNKALVQQMEQLRQKLGPHAGDVEGHLSFLEKDLRDK VNSFFSTFKEKESQDKTLSLPELEQQQEQQQEQQQEQVQMLAPLES

      • Predicted molecular weight

        72 kDa including tags

      • Amino acids

        1 to 396

    Specifications

    Our Abpromise guarantee covers the use of ab132653 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      SDS-PAGE

      Western blot

    • Form

      Liquid

    • Additional notes

      This product was previously labelled as APOA4.

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • Apo AIV
      • APOA 4
      • ApoA IV
      • APOA4
      • Apolipoprotein A IV
      • Apolipoprotein A4
      • MGC142154
      • MGC142156
      see all

    • Relevance

      APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties

    • Cellular localization

      Secreted

    Images

    • SDS-PAGE - Recombinant Human APOA4/Apo-AIV protein (ab132653)
      SDS-PAGE - Recombinant Human APOA4/Apo-AIV protein (ab132653)
      12.5% SDS-PAGE analysis of ab132653 stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab132653? Please let us know so that we can cite the reference in this datasheet.

    ab132653 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Our lab will be needing to order purified apoa4 in the near future. Could you tell me how long it would take to receive this item (ab132653) once it is ordered? We Our intent is to use it in in vitro tissue culture – is the protein you provide active and suitable for this purpose?

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    Answer



    We don't currently have this protein in stock, but once it's ordered, it will take between 2 and 3 weeks to arrive.

    At this time we have not tested the functionality of the protein and we do not know if it is an active protein. It has been used as a control in Western blot and ELISA, but not in tissue culture.

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