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    recombinant-human-apolipoprotein-e4-ab50243.pdf

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Recombinant Human Apolipoprotein E4 (ab50243)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (5)

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Western blot - Recombinant Human Apolipoprotein E4  (ab50243)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Suitable for: SDS-PAGE, WB

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    Description

    • Product name

      Recombinant Human Apolipoprotein E4
      See all Apolipoprotein E4 proteins and peptides
    • Purity

      > 90 % SDS-PAGE.
      ab50243 purity is greater than 90% by SDS-PAGE gel and HPLC analyses.
    • Endotoxin level

      < 1.000 Eu/µg
    • Expression system

      Escherichia coli
    • Accession

      P02649
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MKVEQAVETE PEPELRQQTE WQSGQRWELA LGRFWDYLRW VQTLSEQVQE ELLSSQVTQE LRALMDETMK ELKAYKSELE EQLTPVAEET RARLSKELQA AQARLGADME DVRGRLVQYR GEVQAMLGQS TEELRVRLAS HLRKLRKRLL RDADDLQKRL AVYQAGAREG AERGLSAIRE RLGPLVEQGR VRAATVGSLA GQPLQERAQA WGERLRARME EMGSRTRDRL DEVKEQVAEV RAKLEEQAQQ IRLQAEAFQA RLKSWFEPLV EDMQRQWAGL VEKVQAAVGT SAAPVPSDNH
      • Predicted molecular weight

        34 kDa
      • Amino acids

        19 to 317

    Associated products

    • Related Products

      • Anti-Apolipoprotein E antibody (ab32897)

    Specifications

    Our Abpromise guarantee covers the use of ab50243 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Western blot

    • Form

      Lyophilized
    • Additional notes

      Biological Activity: Data not available.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • Reconstitution
      Centrifuge the vial prior to opening.Reconstitute in water to 0.1-1.0 mg/ml. Allow the reconstituted vial to sit at room temperature for 1 hour before use. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.

    General Info

    • Alternative names

      • AD2
      • Alzheimer disease 2 (APOE*E4 associated, late onset)
      • Apo E4
      • Apo-E
      • APOE
      • APOE_HUMAN
      • apoe4
      • Apolipoprotein E
      • Apolipoprotein E3
      • LDLCQ5
      • LPG
      • MGC1571
      see all
    • Function

      Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
    • Tissue specificity

      Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
    • Involvement in disease

      Defects in APOE are a cause of hyperlipoproteinemia type 3 (HLPP3) [MIM:107741]; also known as familial dysbetalipoproteinemia. Individuals with HLPP3 are clinically characterized by xanthomas, yellowish lipid deposits in the palmar crease, or less specific on tendons and on elbows. The disorder rarely manifests before the third decade in men. In women, it is usually expressed only after the menopause. The vast majority of the patients are homozygous for APOE*2 alleles. More severe cases of HLPP3 have also been observed in individuals heterozygous for rare APOE variants. The influence of APOE on lipid levels is often suggested to have major implications for the risk of coronary artery disease (CAD). Individuals carrying the common APOE*4 variant are at higher risk of CAD.
      Genetic variations in APOE are associated with Alzheimer disease type 2 (AD2) [MIM:104310]. It is a late-onset neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death. Note=The APOE*4 allele is genetically associated with the common late onset familial and sporadic forms of Alzheimer disease. Risk for AD increased from 20% to 90% and mean age at onset decreased from 84 to 68 years with increasing number of APOE*4 alleles in 42 families with late onset AD. Thus APOE*4 gene dose is a major risk factor for late onset AD and, in these families, homozygosity for APOE*4 was virtually sufficient to cause AD by age 80. The mechanism by which APOE*4 participates in pathogenesis is not known.
      Defects in APOE are a cause of sea-blue histiocyte disease (SBHD) [MIM:269600]; also known as sea-blue histiocytosis. This disorder is characterized by splenomegaly, mild thrombocytopenia and, in the bone marrow, numerous histiocytes containing cytoplasmic granules which stain bright blue with the usual hematologic stains. The syndrome is the consequence of an inherited metabolic defect analogous to Gaucher disease and other sphingolipidoses.
      Defects in APOE are a cause of lipoprotein glomerulopathy (LPG) [MIM:611771]. LPG is an uncommon kidney disease characterized by proteinuria, progressive kidney failure, and distinctive lipoprotein thrombi in glomerular capillaries. It mainly affects people of Japanese and Chinese origin. The disorder has rarely been described in Caucasians.
    • Sequence similarities

      Belongs to the apolipoprotein A1/A4/E family.
    • Post-translational
      modifications

      Synthesized with the sialic acid attached by O-glycosidic linkage and is subsequently desialylated in plasma. O-glycosylated with core 1 or possibly core 8 glycans. Thr-307 is a minor glycosylation site compared to Ser-308.
      Glycated in plasma VLDL of normal subjects, and of hyperglycemic diabetic patients at a higher level (2-3 fold).
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02649 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Western blot - Recombinant Human Apolipoprotein E4  (ab50243)
      Western blot - Recombinant Human Apolipoprotein E4 (ab50243)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab50243? Please let us know so that we can cite the reference in this datasheet.

    ab50243 has been referenced in 5 publications.

    • Riad A  et al. The Sigma-2 Receptor/TMEM97, PGRMC1, and LDL Receptor Complex Are Responsible for the Cellular Uptake of Aß42 and Its Protein Aggregates. Mol Neurobiol 57:3803-3813 (2020). PubMed: 32572762
    • Banik SM  et al. Lysosome-targeting chimaeras for degradation of extracellular proteins. Nature 584:291-297 (2020). PubMed: 32728216
    • Khan N  et al. Apolipoprotein E isoform dependently affects Tat-mediated HIV-1 LTR transactivation. J Neuroinflammation 15:91 (2018). PubMed: 29558961
    • Calero O  et al. A fast and cost-effective method for apolipoprotein E isotyping as an alternative to APOE genotyping for patient screening and stratification. Sci Rep 8:5969 (2018). PubMed: 29654261
    • Robert J  et al. Clearance of beta-amyloid is facilitated by apolipoprotein E and circulating high-density lipoproteins in bioengineered human vessels. Elife 6:N/A (2017). PubMed: 28994390

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Question

    Reconstitution d’Apolipoprotéine E4 ?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 09 2012

    Answer

    Merci de nous avoir contactés.

    Suite à notre conversation téléphonique, je peux vous confirmer qu’ab50243 doit être reconstitué dans de l’eau. La protéine a été lyophilisée à partir d’un tampon PBS donc en la reconstituent avec de l’eau, votre protéine sera dans une solution de PBS.

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Vous utilisez nos produits? Partagez vos résultats sous forme d'Abreview et recevez en échange des CADEAUX!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Nov 09 2012

    Question

    Customer kindly called to inquire about the buffer in which these proteins were lyopolized in.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 25 2012

    Answer

    Thank you for contacting us.

    I have contacted the lab to determine what buffers these products were lyopholized in on your behalf. While these buffers can change with lot, the present lots used the following:





    ab50242: Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Sodium Phosphate, pH 7.8 + 0.5 mM DTT.



    ab50243: Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Sodium Phosphate, pH 7.8.



    ab50244: Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Sodium Phosphate, pH 7.8 + 0.5 mM DTT.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Apr 25 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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