Recombinant human ARK5 protein (ab125626)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant human ARK5 protein -
Biological activity
The specific activity of ab125626 was determined to be 50 nmol/min/mg. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
122 kDa including tags -
Amino acids
1 to 661
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab125626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- AMP activated protein kinase family member 5
- AMPK related protein kinase 5
- AMPK-related protein kinase 5
see all -
Function
Involved in tolerance to glucose starvation. Phosphorylates ATM. Suppresses Fas-induced apoptosis by phosphorylation of CASP6, thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR. -
Tissue specificity
Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsUbiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X. - Information by UniProt
Images
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SDS-PAGE - Recombinant human ARK5 protein (ab125626)SDS PAGE analysis of ab125626 -
Functional Studies - Recombinant human ARK5 protein (ab125626)The specific activity of ARK5 (ab125626) was determined to be 54 nmol /min/mg as per activity assay protocol.
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Functional Studies - Recombinant human ARK5 protein (ab125626)The specific activity of ab125626 was determined to be 50 nmol/min/mg. -
SDS-PAGE - Recombinant human ARK5 protein (ab125626)SDS-PAGE analysis of ab125626.
Datasheets and documents
References
ab125626 has not yet been referenced specifically in any publications.
