Recombinant human ARK5 protein (ab125626)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human ARK5 protein -
Biological activity
The specific activity of ab125626 was determined to be 50 nmol/min/mg. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
122 kDa including tags -
Amino acids
1 to 661
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab125626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- AMP activated protein kinase family member 5
- AMPK related protein kinase 5
- AMPK-related protein kinase 5
see all -
Function
Involved in tolerance to glucose starvation. Phosphorylates ATM. Suppresses Fas-induced apoptosis by phosphorylation of CASP6, thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR. -
Tissue specificity
Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsUbiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X. - Information by UniProt
Images
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SDS-PAGE - Recombinant human ARK5 protein (ab125626)SDS PAGE analysis of ab125626
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Functional Studies - Recombinant human ARK5 protein (ab125626)The specific activity of ARK5 (ab125626) was determined to be 54 nmol /min/mg as per activity assay protocol.
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Functional Studies - Recombinant human ARK5 protein (ab125626)The specific activity of ab125626 was determined to be 50 nmol/min/mg. -
SDS-PAGE - Recombinant human ARK5 protein (ab125626)SDS-PAGE analysis of ab125626.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab125626 has not yet been referenced specifically in any publications.
