Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
      O60285
    • Species
      Human
    • Molecular weight
      122 kDa including tags
    • Amino acids
      1 to 661

Associated products

Specifications

Our Abpromise guarantee covers the use of ab125626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab125626 was determined to be 50 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form
    Liquid
  • Additional notes

    ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • AMP activated protein kinase family member 5
    • AMPK related protein kinase 5
    • AMPK-related protein kinase 5
    • NUAK family SNF1 like kinase
    • NUAK family SNF1-like kinase 1
    • Nuak1
    • NUAK1_HUMAN
    • Omphalocele kinase 1
    • Probable serine/threonine protein kinase KIAA0537
    see all
  • Function
    Involved in tolerance to glucose starvation. Phosphorylates ATM. Suppresses Fas-induced apoptosis by phosphorylation of CASP6, thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR.
  • Tissue specificity
    Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human ARK5 protein (ab125626)
    Functional Studies - Recombinant human ARK5 protein (ab125626)
    The specific activity of ab125626 was determined to be 50 nmol/min/mg.
  • SDS-PAGE - Recombinant human ARK5 protein (ab125626)
    SDS-PAGE - Recombinant human ARK5 protein (ab125626)
    SDS-PAGE analysis of ab125626.

References

ab125626 has not yet been referenced specifically in any publications.

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