Recombinant Human ASF1b protein (ab130033)

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Description

  • Product name

    Recombinant Human ASF1b protein

  • Purity

    > 85 % SDS-PAGE.
    ab130033 was purified by using conventional chromatography techniques.

  • Expression system

    Escherichia coli

  • Accession

    Q9NVP2

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTP IKGLGLPGCI PGLLPENSMD CILEHHHHHH

    • Predicted molecular weight

      23 kDa including tags

    • Amino acids

      1 to 202

    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab130033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.88% Sodium chloride, 10% Glycerol, 0.32% Tris HCl, 0.02% DTT

General Info

  • Alternative names

    • Anti silencing function 1B
    • Anti silencing function 1B histone chaperone
    • Anti-silencing function protein 1 homolog B
    • ASF1 anti silencing function 1 homolog B
    • ASF1 anti silencing function 1 homolog B (S. cerevisiae)
    • ASF1B
    • ASF1B_HUMAN
    • CCG1 interacting factor A II
    • CCG1-interacting factor A-II
    • CIA-II
    • hAsf1
    • hAsf1b
    • hCIA-II
    • Histone chaperone ASF1B
    see all

  • Function

    Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.

  • Tissue specificity

    Highly expressed in testis and at lower levels in colon, small intestine and thymus.

  • Sequence similarities

    Belongs to the ASF1 family.

  • Post-translational
    modifications

    Phosphorylated by TLK1 and TLK2.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human ASF1b protein (ab130033)
    SDS-PAGE - Recombinant Human ASF1b protein (ab130033)
    ab130033 at 3 µg analysed by 15% SDS-PAGE.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab130033? Please let us know so that we can cite the reference in this datasheet.

ab130033 has not yet been referenced specifically in any publications.

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