Recombinant Human ASF1b protein (ab130033)

Submit a review Submit a question
SDS-PAGE - Recombinant Human ASF1b protein (ab130033)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag C-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human ASF1b protein

    • Purity

      > 85 % SDS-PAGE.
      ab130033 was purified by using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      Q9NVP2

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTP IKGLGLPGCI PGLLPENSMD CILEHHHHHH

      • Predicted molecular weight

        23 kDa including tags

      • Amino acids

        1 to 202

      • Tags

        His tag C-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab130033 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.88% Sodium chloride, 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% DTT

    General Info

    • Alternative names

      • Anti silencing function 1B
      • Anti silencing function 1B histone chaperone
      • Anti-silencing function protein 1 homolog B
      • ASF1 anti silencing function 1 homolog B
      • ASF1 anti silencing function 1 homolog B (S. cerevisiae)
      • ASF1B
      • ASF1B_HUMAN
      • CCG1 interacting factor A II
      • CCG1-interacting factor A-II
      • CIA-II
      • hAsf1
      • hAsf1b
      • hCIA-II
      • Histone chaperone ASF1B
      see all

    • Function

      Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.

    • Tissue specificity

      Highly expressed in testis and at lower levels in colon, small intestine and thymus.

    • Sequence similarities

      Belongs to the ASF1 family.

    • Post-translational
      modifications

      Phosphorylated by TLK1 and TLK2.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human ASF1b protein (ab130033)
      SDS-PAGE - Recombinant Human ASF1b protein (ab130033)
      ab130033 at 3 µg analysed by 15% SDS-PAGE.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab130033? Please let us know so that we can cite the reference in this datasheet.

    ab130033 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab130033.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com