Recombinant Human ASF1b protein (ab130033)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Human ASF1b protein -
Purity
> 85 % SDS-PAGE.
ab130033 was purified by using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTP IKGLGLPGCI PGLLPENSMD CILEHHHHHH -
Predicted molecular weight
23 kDa including tags -
Amino acids
1 to 202 -
Tags
His tag C-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab130033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.88% Sodium chloride, 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% DTT
General Info
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Alternative names
- Anti silencing function 1B
- Anti silencing function 1B histone chaperone
- Anti-silencing function protein 1 homolog B
see all -
Function
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis. -
Tissue specificity
Highly expressed in testis and at lower levels in colon, small intestine and thymus. -
Sequence similarities
Belongs to the ASF1 family. -
Post-translational
modificationsPhosphorylated by TLK1 and TLK2. -
Cellular localization
Nucleus. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human ASF1b protein (ab130033)ab130033 at 3 µg analysed by 15% SDS-PAGE.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab130033 has not yet been referenced specifically in any publications.