Recombinant Human ASF1b protein (ab130033)

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Overview

  • Product name

    Recombinant Human ASF1b protein

  • Protein length

    Full length protein

Description

  • Nature

    Recombinant

  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
      Q9NVP2

    • Species

      Human

    • Sequence

      MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTP IKGLGLPGCI PGLLPENSMD CILEHHHHHH

    • Molecular weight

      23 kDa including tags

    • Amino acids

      1 to 202

    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab130033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF

  • Purity

    > 85 % SDS-PAGE.
    ab130033 was purified by using conventional chromatography techniques.

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names

    • Anti silencing function 1B
    • Anti silencing function 1B histone chaperone
    • Anti-silencing function protein 1 homolog B
    • ASF1 anti silencing function 1 homolog B
    • ASF1 anti silencing function 1 homolog B (S. cerevisiae)
    • ASF1B
    • ASF1B_HUMAN
    • CCG1 interacting factor A II
    • CCG1-interacting factor A-II
    • CIA-II
    • hAsf1
    • hAsf1b
    • hCIA-II
    • Histone chaperone ASF1B
    see all

  • Function

    Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.

  • Tissue specificity

    Highly expressed in testis and at lower levels in colon, small intestine and thymus.

  • Sequence similarities

    Belongs to the ASF1 family.

  • Post-translational
    modifications

    Phosphorylated by TLK1 and TLK2.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human ASF1b protein (ab130033)
    SDS-PAGE - Recombinant Human ASF1b protein (ab130033)
    ab130033 at 3 µg analysed by 15% SDS-PAGE.

References

ab130033 has not yet been referenced specifically in any publications.

Publishing research using ab130033? Please let us know so that we can cite the reference in this datasheet.

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