Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Human ATG14L protein (Tagged)
Purity> 90 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMASPSGKGARALEAPGCGPRPLARDLVDSVDDAEGLYVAVERCPLCNTTR RRLTCAKCVQSGDFVYFDGRDRERFIDKKERLSRLKSKQEEFQKEVLKAM EGKWITDQLRWKIMSCKMRIEQLKQTICKGNEEMEKNSEGLLKTKEKNQK LYSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLRSHYERLANLRRSHILE LTSVIFPIEEVKTGVRDPADVSSESDSAMTSSTVSKLAEARRTTYLSGRW VCDDHNGDTSISITGPWISLPNNGDYSAYYSWVEEKKTTQGPDMEQSNPA YTISAALCYATQLVNILSHILDVNLPKKLCNSEFCGENLSKQKFTRAVKK LNANILYLCFSQHVNLDQLQPLHTLRNLMYLVSPSSEHLGRSGPFEVRAD LEESMEFVDPGVAGESDESGDERVSDEETDLGTDWENLPSPRFCDIPSQS VEVSQSQSTQASPPIASSSAGGMISSAAASVTSWFKAYTGHR
Predicted molecular weight60 kDa including tags
Amino acids1 to 492
TagsHis tag N-Terminus
Additional sequence information10xHis tag at the N-terminus and a Myc tag at the C-terminus.
- Anti-ATG14L antibody (ab139727)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Myc tag antibody [Myc.A7] (ab18185)
- Anti-ATG14L antibody (ab189949)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Myc tag antibody (ab9106)
- Anti-6X His tag® antibody (ab9108)
- Anti-Myc tag antibody - ChIP Grade (ab9132)
Our Abpromise guarantee covers the use of ab225977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 50% Glycerol (glycerin, glycerine), Tris buffer
FunctionRequired for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.
Sequence similaritiesBelongs to the Barkor family.
DomainThe coiled-coil domain is required for BECN1- and PIK3C3-binding and for autophagy.
Cellular localizationCytoplasm. Endoplasmic reticulum. Cytosolic under nutrient-rich conditions. Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225977 has not yet been referenced specifically in any publications.