Recombinant Human ATG4A protein (ab172826)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Tags: His tag N-Terminus
  • Suitable for: HPLC, SDS-PAGE

Description

  • Product name

    Recombinant Human ATG4A protein

  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Supplied as a 0.2 µM filtered solution.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    Q8WYN0

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMESVLSKYEDQITIFTDYLEEYPDTDELVW ILGKQHLLKTEKSKLLSDISARLWFTYRRKFSPIGGTGPSSDAGWGCMLR CGQMMLAQALICRHLGRDWSWEKQKEQPKEYQRILQCFLDRKDCCYSIHQ MAQMGVGEGKSIGEWFGPNTVAQVLKKLALFDEWNSLAVYVSMDNTVVIE DIKKMCRVLPLSADTAGDRPPDSLTASNQSKGTSAYCSAWKPLLLIVPLR LGINQINPVYVDAFKECFKMPQSLGALGGKPNNAYYFIGFLGDELIFLDP HTTQTFVDTEENGTVNDQTFHCLQSPQRMNILNLDPSVALGFFCKEEKDF DNWCSLVQKEILKENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEFIDS TEQLEEFDLEEDFEILSV

    • Predicted molecular weight

      45 kDa

    • Amino acids

      1 to 398

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab172826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 100% PBS

General Info

  • Alternative names

    • AI627006
    • APG4 autophagy 4 homolog A
    • Apg4a
    • ATG4 autophagy related 4 homolog A
    • ATG4 autophagy related 4 homolog A (S. cerevisiae)
    • ATG4A
    • ATG4A_HUMAN
    • Atg4al
    • AUT like 2 cysteine endopeptidase
    • AUT-like 2 cysteine endopeptidase
    • Autl2
    • Autophagin 2
    • Autophagin-2
    • Autophagy related 4A cysteine peptidase
    • Autophagy related cysteine endopeptidase 2
    • Autophagy related protein 4 homolog A
    • Autophagy-related cysteine endopeptidase 2
    • Autophagy-related protein 4 homolog A
    • AV169859
    • Cysteine protease ATG4A
    • hAPG4A
    • MGC107179
    see all

  • Function

    Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.

  • Tissue specificity

    Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.

  • Sequence similarities

    Belongs to the peptidase C54 family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab172826? Please let us know so that we can cite the reference in this datasheet.

ab172826 has not yet been referenced specifically in any publications.

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