Description

  • Product name

    Recombinant human ATP1B1 protein (Active)
  • Biological activity

    Specific activity is > 3,000 pmol/min/µg, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-triphosphate to phosphate per minute per minute at pH 7.5 at 25°C.

  • Purity

    > 90 % SDS-PAGE.
    ab222961 was purified by using conventional chromatography techniques.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Baculovirus infected Sf21 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      ADPEFKPTYQ DRVAPPGLTQ IPQIQKTEIS FRPNDPKSYE AYVLNIVRFL EKYKDSAQRD DMIFEDCGDV PSEPKERGDF NHERGERKVC RFKLEWLGNC SGLNDETYGY KEGKPCIIIK LNRVLGFKPK PPKNESLETY PVMKYNPNVL PVQCTGKRDE DKDKVGNVEY FGLGNSPGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTMD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKSHHHHHH
    • Predicted molecular weight

      29 kDa including tags
    • Amino acids

      63 to 303
    • Tags

      His tag C-Terminus
    • Additional sequence information

      Extracellular domain.

Specifications

Our Abpromise guarantee covers the use of ab222961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Additional notes

    Background reference: PMID 26506237.

    Shi et al. Overexpression of ATP1B1 predicts an adverse prognosis in cytogenetically normal acute myeloid leukemia. Oncotarget. 2016 Jan 19;7(3):2585-95. doi: 10.18632/oncotarget.6226.

     This product was previously labelled as beta 1 Sodium Potassium ATPase

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.4
    Constituents: 10% Glycerol, PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Adenosinetriphosphatase
    • AT1B1_HUMAN
    • ATP 1B
    • ATP1B
    • ATP1B1
    • ATPase Na+/K+ transporting beta 1 polypeptide
    • Atpb 1
    • ATPBS
    • Beta 1 subunit of Na(+) K(+) ATPase
    • Na K ATPase beta 1 polypeptide
    • Na+/K+ ATPase beta 1 subunit
    • Sodium/potassium dependent ATPase beta 1 subunit
    • Sodium/potassium transporting ATPase beta 1 chain
    • Sodium/potassium transporting ATPase subunit beta 1
    • Sodium/potassium-dependent ATPase subunit beta-1
    • Sodium/potassium-transporting ATPase subunit beta-1
    see all
  • Function

    This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
  • Tissue specificity

    Found in most tissues.
  • Sequence similarities

    Belongs to the X(+)/potassium ATPases subunit beta family.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human ATP1B1 protein (Active) (3 µg ab222961).

    28-40 KDa under reducing conditions.

References

ab222961 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222961.
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