Key features and details
- Expression system: Baculovirus infected Sf21 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human ATP1B1 protein (Active)
Specific activity is > 3,000 pmol/min/µg, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-triphosphate to phosphate per minute per minute at pH 7.5 at 25°C.
Purity> 90 % SDS-PAGE.
ab222961 was purified by using conventional chromatography techniques.
Endotoxin level< 1.000 Eu/µg
Expression systemBaculovirus infected Sf21 cells
Protein lengthProtein fragment
SequenceADPEFKPTYQ DRVAPPGLTQ IPQIQKTEIS FRPNDPKSYE AYVLNIVRFL EKYKDSAQRD DMIFEDCGDV PSEPKERGDF NHERGERKVC RFKLEWLGNC SGLNDETYGY KEGKPCIIIK LNRVLGFKPK PPKNESLETY PVMKYNPNVL PVQCTGKRDE DKDKVGNVEY FGLGNSPGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTMD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKSHHHHHH
Predicted molecular weight29 kDa including tags
Amino acids63 to 303
TagsHis tag C-Terminus
Additional sequence informationExtracellular domain.
Our Abpromise guarantee covers the use of ab222961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Background reference: PMID 26506237.
Shi et al. Overexpression of ATP1B1 predicts an adverse prognosis in cytogenetically normal acute myeloid leukemia. Oncotarget. 2016 Jan 19;7(3):2585-95. doi: 10.18632/oncotarget.6226.
This product was previously labelled as beta 1 Sodium Potassium ATPase
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 10% Glycerol (glycerin, glycerine), PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ATP 1B
FunctionThis is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Tissue specificityFound in most tissues.
Sequence similaritiesBelongs to the X(+)/potassium ATPases subunit beta family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab222961 has not yet been referenced specifically in any publications.