Recombinant human ATP1B1 protein (Active) (ab222961)
- Datasheet
- References
- Protocols
Description
-
Product name
Recombinant human ATP1B1 protein (Active) -
Biological activity
Specific activity is > 3,000 pmol/min/µg, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-triphosphate to phosphate per minute per minute at pH 7.5 at 25°C.
-
Purity
> 90 % SDS-PAGE.
ab222961 was purified by using conventional chromatography techniques. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Baculovirus infected Sf21 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
ADPEFKPTYQ DRVAPPGLTQ IPQIQKTEIS FRPNDPKSYE AYVLNIVRFL EKYKDSAQRD DMIFEDCGDV PSEPKERGDF NHERGERKVC RFKLEWLGNC SGLNDETYGY KEGKPCIIIK LNRVLGFKPK PPKNESLETY PVMKYNPNVL PVQCTGKRDE DKDKVGNVEY FGLGNSPGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTMD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKSHHHHHH -
Predicted molecular weight
29 kDa including tags -
Amino acids
63 to 303 -
Tags
His tag C-Terminus -
Additional sequence information
Extracellular domain.
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab222961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
SDS-PAGE
-
Additional notes
Background reference: PMID 26506237.
Shi et al. Overexpression of ATP1B1 predicts an adverse prognosis in cytogenetically normal acute myeloid leukemia. Oncotarget. 2016 Jan 19;7(3):2585-95. doi: 10.18632/oncotarget.6226.
This product was previously labelled as beta 1 Sodium Potassium ATPase
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 10% Glycerol, PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- Adenosinetriphosphatase
- AT1B1_HUMAN
- ATP 1B
see all -
Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. -
Tissue specificity
Found in most tissues. -
Sequence similarities
Belongs to the X(+)/potassium ATPases subunit beta family. -
Cellular localization
Membrane. - Information by UniProt
Images
-
SDS-PAGE - Recombinant human ATP1B1 protein (Active) (ab222961)SDS-PAGE - Recombinant human ATP1B1 protein (Active) (3 µg ab222961).
28-40 KDa under reducing conditions.
Datasheets and documents
References
ab222961 has not yet been referenced specifically in any publications.
