Bioactive grade

Recombinant human ATP1B1 protein (Active) (ab222961)

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SDS-PAGE - Recombinant human ATP1B1 protein (Active) (ab222961)

    Key features and details

    • Expression system: Baculovirus infected Sf21 cells
    • Purity: > 90% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Tags: His tag C-Terminus
    • Suitable for: Functional Studies, SDS-PAGE

    Description

    • Product name

      Recombinant human ATP1B1 protein (Active)
      See all ATP1B1 proteins and peptides

    • Biological activity

      Specific activity is > 3,000 pmol/min/µg, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-triphosphate to phosphate per minute per minute at pH 7.5 at 25°C.

    • Purity

      > 90 % SDS-PAGE.
      Affinity purified

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      Baculovirus infected Sf21 cells

    • Accession

      P05026

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        ADPEFKPTYQ DRVAPPGLTQ IPQIQKTEIS FRPNDPKSYE AYVLNIVRFL EKYKDSAQRD DMIFEDCGDV PSEPKERGDF NHERGERKVC RFKLEWLGNC SGLNDETYGY KEGKPCIIIK LNRVLGFKPK PPKNESLETY PVMKYNPNVL PVQCTGKRDE DKDKVGNVEY FGLGNSPGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTMD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKSHHHHHH

      • Predicted molecular weight

        29 kDa including tags

      • Amino acids

        63 to 303

      • Tags

        His tag C-Terminus

      • Additional sequence information

        Extracellular domain.

    Specifications

    Our Abpromise guarantee covers the use of ab222961 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

    • Additional notes

      Background reference: PMID 26506237.

      Shi et al. Overexpression of ATP1B1 predicts an adverse prognosis in cytogenetically normal acute myeloid leukemia. Oncotarget. 2016 Jan 19;7(3):2585-95. doi: 10.18632/oncotarget.6226.

       This product was previously labelled as beta 1 Sodium Potassium ATPase

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituents: 10% Glycerol (glycerin, glycerine), PBS

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • Adenosinetriphosphatase
      • AT1B1_HUMAN
      • ATP 1B
      • ATP1B
      • ATP1B1
      • ATPase Na+/K+ transporting beta 1 polypeptide
      • Atpb 1
      • ATPBS
      • Beta 1 subunit of Na(+) K(+) ATPase
      • Na K ATPase beta 1 polypeptide
      • Na+/K+ ATPase beta 1 subunit
      • Sodium/potassium dependent ATPase beta 1 subunit
      • Sodium/potassium transporting ATPase beta 1 chain
      • Sodium/potassium transporting ATPase subunit beta 1
      • Sodium/potassium-dependent ATPase subunit beta-1
      • Sodium/potassium-transporting ATPase subunit beta-1
      see all

    • Function

      This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.

    • Tissue specificity

      Found in most tissues.

    • Sequence similarities

      Belongs to the X(+)/potassium ATPases subunit beta family.

    • Cellular localization

      Membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human ATP1B1 protein (Active) (ab222961)
      SDS-PAGE - Recombinant human ATP1B1 protein (Active) (ab222961)

      SDS-PAGE - Recombinant human ATP1B1 protein (Active) (3 µg ab222961).

      28-40 KDa under reducing conditions.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab222961? Please let us know so that we can cite the reference in this datasheet.

    ab222961 has not yet been referenced specifically in any publications.

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