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  1. Link

    recombinant-human-atp5a-protein-tagged-ab235845.pdf

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Recombinant Human ATP5A protein (Tagged) (ab235845)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant Human ATP5A protein (Tagged) (ab235845)
  • Mass Spectrometry - Recombinant Human ATP5A protein (Tagged) (ab235845)
  • Mass Spectrometry - Recombinant Human ATP5A protein (Tagged) (ab235845)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: MS, SDS-PAGE

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Description

  • Product name

    Recombinant Human ATP5A protein (Tagged)
    See all ATP5A proteins and peptides
  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

    P25705
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      QKTGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAE EMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVG EELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGI KAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLY CIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSG CSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFY LHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQI FLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREV AAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVR GYLDKLEPSKITKFENAFLSHVVSQHQALLGTIRADGKISEQSDAKLKEI VTNFLAGFEA
    • Predicted molecular weight

      71 kDa including tags
    • Amino acids

      44 to 553
    • Tags

      His tag N-Terminus
    • Additional sequence information

      Full-length mature chain lacking the transit petide. N-terminal 6xHis-SUMO-tagged.

Associated products

  • Compatible Secondaries

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-6X His tag® antibody (ab9108)
  • Related Products

    • Anti-ATP5A antibody (ab151229)
    • Anti-ATP5A antibody [EPR13030(B)] (ab176569)

Specifications

Our Abpromise guarantee covers the use of ab235845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    LC-MS/MS
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.2
    Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

General Info

  • Alternative names

    • ATP synthase alpha chain
    • ATP synthase alpha chain, mitochondrial
    • ATP synthase subunit alpha
    • ATP synthase subunit alpha mitochondrial
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2
    • ATP sythase (F1 ATPase) alpha subunit
    • ATP5A
    • Atp5a1
    • ATP5AL2
    • ATPA_HUMAN
    • ATPM
    • Epididymis secretory sperm binding protein Li 123m
    • hATP1
    • HEL-S-123m
    • MC5DN4
    • mitochondrial
    • Mitochondrial ATP synthetase
    • Mitochondrial ATP synthetase oligomycin resistant
    • Modifier of Min 2
    • Modifier of Min 2 mouse homolog
    • Modifier of Min 2, mouse, homolog of
    • MOM2
    • OMR
    • ORM
    • OTTHUMP00000163475
    see all
  • Function

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
  • Tissue specificity

    Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
  • Sequence similarities

    Belongs to the ATPase alpha/beta chains family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Mitochondrion inner membrane. Peripheral membrane protein.
  • Target information above from: UniProt accession P25705 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant Human ATP5A protein (Tagged) (ab235845)
    SDS-PAGE - Recombinant Human ATP5A protein (Tagged) (ab235845)

    ab235845 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

  • Mass Spectrometry - Recombinant Human ATP5A protein (Tagged) (ab235845)
    Mass Spectrometry - Recombinant Human ATP5A protein (Tagged) (ab235845)

    Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab235845 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ATP5A.

  • Mass Spectrometry - Recombinant Human ATP5A protein (Tagged) (ab235845)
    Mass Spectrometry - Recombinant Human ATP5A protein (Tagged) (ab235845)

    Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab235845 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ATP5A.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab235845? Please let us know so that we can cite the reference in this datasheet.

ab235845 has not yet been referenced specifically in any publications.

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