Recombinant Human ATR protein (ab204140)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% SDS-PAGE
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human ATR protein -
Purity
> 85 % SDS-PAGE.
Assessed by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MLKKLVEEATFSEILIPLQSVMIPTLPSILGTHANHASHEPFPGHWAYIA GFDDMVEILASLQKPKKISLKGSDGKFYIMMCKPKDDLRKDCRLMEFNSL INKCLRKDAESRRRELHIRTYAVIPLNDECGIIEWVNNTAGLRPILTKLY KEKGVYMTGKELRQCMLPKSAALSEKLKVFREFLLPRHPPIFHEWFLRTF PDPTSWYSSRSAYCRSTAVMSMVGYILGLGDRHGENILFDSLTGECVHVD FNCLFNKGETFEVPEIVPFRLTHNMVNGMGPMGTEGLFRRACEVTMRLMR DQREPLMSVLKTFLHDPLVEWSKPVKGHSKAPLNETGEVVNEKAKTHVLD IEQRLQGVIK TRNRVT -
Predicted molecular weight
70 kDa including tags -
Amino acids
2245 to 2610 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab204140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- Ataxia telangiectasia and Rad3 related
- Ataxia telangiectasia and Rad3-related protein
- ATR
see all -
Function
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. -
Tissue specificity
Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary. -
Involvement in disease
Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. -
Sequence similarities
Belongs to the PI3/PI4-kinase family. ATM subfamily.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain. -
Post-translational
modificationsPhosphorylated; autophosphorylates in vitro. -
Cellular localization
Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab204140 has not yet been referenced specifically in any publications.