Description

  • Product name

    Recombinant human Aurora A protein
    See all Aurora A proteins and peptides
  • Biological activity

    120 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30?C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.5 mM ATP, 4 uM aurora A substrate, and 0.4 ug/ml Aurora A kinase.
  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Amino acids

      2 to 403
    • Tags

      His tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab42595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Inhibition Assay

  • Form

    Liquid
  • Additional notes

    Expressed in Baculovirus infected Sf9 cell expression system.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.0462% DTT, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • AIK
    • ARK-1
    • ARK1
    • AURA
    • Aurka
    • Aurora 2
    • Aurora A
    • Aurora kinase A
    • Aurora-related kinase 1
    • Aurora/IPL1 like kinase
    • AURORA/IPL1-like kinase
    • Aurora/IPL1-related kinase 1
    • AURORA2
    • Breast tumor-amplified kinase
    • BTAK
    • hARK1
    • IAK
    • IPL1 related kinase
    • MGC34538
    • OTTHUMP00000031340
    • OTTHUMP00000031341
    • OTTHUMP00000031342
    • OTTHUMP00000031343
    • OTTHUMP00000031344
    • OTTHUMP00000031345
    • OTTHUMP00000166071
    • OTTHUMP00000166072
    • PPP1R47
    • Protein phosphatase 1, regulatory subunit 47
    • Serine/threonine kinase 15
    • Serine/threonine kinase 6
    • Serine/threonine protein kinase 15
    • Serine/threonine-protein kinase 15
    • Serine/threonine-protein kinase 6
    • Serine/threonine-protein kinase aurora-A
    • STK15
    • STK6
    • STK6_HUMAN
    • STK7
    see all
  • Function

    Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
  • Tissue specificity

    Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
  • Information by UniProt

References

ab42595 has not yet been referenced specifically in any publications.

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