Key features and details
- Expression system: HEK 293 cells
- Purity: >= 75% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human Aurora A protein (Active)
See all Aurora A proteins and peptides
Biological activitySpecific Activity; 45 pmole/min/μg.
Recombinant human Aurora A protein activity was 45 pmole/min/μg in a kinase assay using KEMPtide as substrate (0.2 mg/ml), containing 2 mM DTT using and 10 μM ATP in assay buffer. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using ADP-Glo reagent.
Purity>= 75 % SDS-PAGE.
Expression systemHEK 293 cells
Protein lengthFull length protein
SequenceDRSKENCISG PVKATAPVGG PKRVLVTQQF PCQNPLPVNS GQAQRVLCPS NSSQRVPLQA QKLVSSHKPV QNQKQKQLQA TSVPHPVSRP LNNTQKSKQP LPSAPENNPE EELASKQKNE ESKKRQWALE DFEIGRPLGK GKFGNVYLAR EKQSKFILAL KVLFKAQLEK AGVEHQLRRE VEIQSHLRHP NILRLYGYFH DATRVYLILE YAPLGTVYRE LQKLSKFDEQ RTATYITELA NALSYCHSKR VIHRDIKPEN LLLGSAGELK IADFGWSVHA PSSRRTTLCG TLDYLPPEMI EGRMHDEKVD LWSLGVLCYE FLVGKPPFEA NTYQETYKRI SRVEFTFPDF VTEGARDLIS RLLKHNPSQR PMLREVLEHP WITANSSKPS NCQNKESASK QS
Predicted molecular weight46 kDa including tags
Amino acids2 to 403
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab271368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 1.36% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
FunctionContributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
Tissue specificityHighly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
modificationsActivated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271368 has not yet been referenced specifically in any publications.