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  1. Link

    recombinant-human-aurora-b-protein-ab51435.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Aurora
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Recombinant human Aurora B protein (ab51435)

  • Datasheet
Submit a review Submit a question References (2)

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SDS-PAGE - Recombinant human Aurora B protein (ab51435)
  • Functional Studies - Recombinant human Aurora B protein (ab51435)
  • Functional Studies - Recombinant human Aurora B protein (ab51435)
  • SDS-PAGE - Recombinant human Aurora B protein (ab51435)
  • Western blot - Recombinant human Aurora B protein (ab51435)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 70% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE, WB

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Description

  • Product name

    Recombinant human Aurora B protein
    See all Aurora B proteins and peptides
  • Purity

    > 70 % Densitometry.
    Affinity purified.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Tags

      GST tag N-Terminus

Associated products

  • Related Products

    • Anti-Aurora B antibody (ab2254)
    • Anti-Aurora B antibody [mAbcam 3609] (ab3609)
    • Anti-Aurora B antibody [EP1009Y] (ab45145)
  • Substrate reagent

    • Recombinant Human Myelin Basic Protein (Tagged) (ab43614)

Specifications

Our Abpromise guarantee covers the use of ab51435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Additional notes

    ab51435 was expressed in Sf9 insect cells.

    ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • AIK2
    • AIM-1
    • AIM1
    • ARK-2
    • ARK2
    • AurB
    • AURKB
    • AURKB_HUMAN
    • Aurora 1
    • Aurora and Ipl1 like midbody associated protein 1
    • Aurora kinase B
    • Aurora related kinase 2
    • Aurora- and Ipl1-like midbody-associated protein 1
    • Aurora-B
    • Aurora-related kinase 2
    • Aurora/IPL1 related kinase 2
    • Aurora/IPL1-related kinase 2
    • IPL1
    • PPP1R48
    • Protein phosphatase 1 regulatory subunit 48
    • Serine/theronine kinase 12
    • Serine/threonine protein kinase 12
    • Serine/threonine-protein kinase 12
    • Serine/threonine-protein kinase aurora-B
    • STK-1
    • STK1
    • STK12
    • STK5
    see all
  • Function

    May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
  • Tissue specificity

    High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
  • Involvement in disease

    Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
  • Target information above from: UniProt accession Q96GD4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant human Aurora B protein (ab51435)
    SDS-PAGE - Recombinant human Aurora B protein (ab51435)
    SDS PAGE analysis of ab51435
  • Functional Studies - Recombinant human Aurora B protein (ab51435)
    Functional Studies - Recombinant human Aurora B protein (ab51435)

    The specific activity of Aurora B (ab51435) was determined to be 19.6 nmol/min/mg as per activity assay protocol and was equivalent to 189.2 nmol/min/mg as per radiometric assay.

  • Functional Studies - Recombinant human Aurora B protein (ab51435)
    Functional Studies - Recombinant human Aurora B protein (ab51435)
    Kinase activity assay - specific activity 190nmol/min/mg
  • SDS-PAGE - Recombinant human Aurora B protein (ab51435)
    SDS-PAGE - Recombinant human Aurora B protein (ab51435)
    Recombinant human Aurora B (tagged). Molecular weight 68 kDa.
  • Western blot - Recombinant human Aurora B protein (ab51435)
    Western blot - Recombinant human Aurora B protein (ab51435)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab51435? Please let us know so that we can cite the reference in this datasheet.

ab51435 has been referenced in 2 publications.

  • Bhowmick R  et al. The RIF1-PP1 Axis Controls Abscission Timing in Human Cells. Curr Biol 29:1232-1242.e5 (2019). PubMed: 30905608
  • Morrison EA  et al. The conformation of the histone H3 tail inhibits association of the BPTF PHD finger with the nucleosome. Elife 7:N/A (2018). PubMed: 29648537

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