Recombinant Human B2M + FCGRT (biotinylated ) protein (ab207117)

Description

  • Product name

    Recombinant Human B2M + FCGRT (biotinylated ) protein
    See all B2M + FCGRT proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Amino Acid Sequence 1

    • Species

      Human
    • Sequence

      AESHLSLLYHLTAVSSPAPGTPAFWVSGWLGPQQYLSYNSLRGEAEPCGA WVWENQVSWYWEKETTDLRIKEKLFLEAFKALGGKGPYTLQGLLGCELGP DNTSVPTAKFALNGEEFMNFDLKQGTWGGDWPEALAISQRWQQQDKAANK ELTFLLFSCPHRLREHLERGRGNLEWKEPPSMRLKARPSSPGFSVLTCSA FSFYPPELQLRFLRNGLAAGTGQGDFGPNSDGSFHASSSLTVKSGDEHHY CCIVQHAGLAQPLRVELESPAKSS
    • Predicted molecular weight

      33 kDa including tags
    • Amino acids

      24 to 297
    • Tags

      His tag C-Terminus , Avi tag C-Terminus
    • Additional sequence information

      Extracellular domain (NP_001129491.1).
    • Amino Acid Sequence 2

    • Species

      Human
    • Sequence

      IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVE HSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
    • Predicted molecular weight

      13 kDa including tags
    • Amino acids

      21 to 119
    • Tags

      StrepII tag C-Terminus
    • Additional sequence information

      This product is the mature full length B2M protein from aa 21 to 119. The signal peptide is not included (AAH32589).

Specifications

Our Abpromise guarantee covers the use of ab207117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    ab207117 B2M + FCGRT heterodimer was produced by co-expression of FCGRT and B2M and enzymatically single-point biotin-labeled at C-terminus to retain its native conformational structure and bioactivity.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    pH: 7.40
    Constituents: 95% PBS, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.

  • Reconstitution
    Reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • beta chain of MHC class I molecules
    • beta-2-microglobin
    • Beta-2-microglobulin
    • c fragment of IgG, receptor, transporter, alpha
    • Fc Fragment of IgG, receptor transporter, alpha
    • FcRn
    • FcRn alpha chain
    • IgG Fc fragment receptor transporter alpha chain
    • IgG receptor FcRn large subunit p51
    • immunoglobulin receptor, intestinal, heavy chain
    • major histocompatibility complex class I-like Fc receptor
    • Neonatal Fc receptor
    • neonatal Fc-receptor for Ig
    see all
  • Relevance

    FCGRT + B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT has a possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
  • Cellular localization

    FCGRT: Cell membrane; Single-pass type I membrane protein B2M: Secreted

Images

  • DTT-reduced SDS-PAGE analysis of ab207117 stained overnight with Coomassie Blue.

    DTT-reduced protein migrates as 36 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.

References

ab207117 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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